Root phenomics of crops: opportunities and challenges
- 1 January 2009
- journal article
- research article
- Published by CSIRO Publishing in Functional Plant Biology
- Vol. 36 (11), 922-929
- https://doi.org/10.1071/fp09150
Abstract
Reliable techniques for screening large numbers of plants for root traits are still being developed, but include aeroponic, hydroponic and agar plate systems. Coupled with digital cameras and image analysis software, these systems permit the rapid measurement of root numbers, length and diameter in moderate (typically <1000) numbers of plants. Usually such systems are employed with relatively small seedlings, and information is recorded in 2D. Recent developments in X-ray microtomography have facilitated 3D non-invasive measurement of small root systems grown in solid media, allowing angular distributions to be obtained in addition to numbers and length. However, because of the time taken to scan samples, only a small number can be screened (typically <10 per day, not including analysis time of the large spatial datasets generated) and, depending on sample size, limited resolution may mean that fine roots remain unresolved. Although agar plates allow differences between lines and genotypes to be discerned in young seedlings, the rank order may not be the same when the same materials are grown in solid media. For example, root length of dwarfing wheat (Triticum aestivum L.) lines grown on agar plates was increased by ~40% relative to wild-type and semi-dwarfing lines, but in a sandy loam soil under well watered conditions it was decreased by 24–33%. Such differences in ranking suggest that significant soil environment–genotype interactions are occurring. Developments in instruments and software mean that a combination of high-throughput simple screens and more in-depth examination of root–soil interactions is becoming viable.Keywords
This publication has 39 references indexed in Scilit:
- EZ‐Rhizo: integrated software for the fast and accurate measurement of root system architectureThe Plant Journal, 2009
- Plant growth habit, root architecture traits and tolerance to low soil phosphorus in an Andean bean populationEuphytica, 2008
- Root system architecture: opportunities and constraints for genetic improvement of cropsTrends in Plant Science, 2007
- The effect of semi-dwarf genes on root system size in field-grown barleyTheoretical and Applied Genetics, 2006
- Root responses to soil physical conditions; growth dynamics from field to cellJournal of Experimental Botany, 2005
- Gel observation chamber for rapid screening of root traits in cereal seedlingsPlant and Soil, 2004
- Effect of phosphorus deficiency on growth angle of basal roots in Phaseolus vulgarisNew Phytologist, 1996
- In-situ root extent measurements by electrical capacitance methodsPlant and Soil, 1995
- Characterization of the Least Limiting Water Range of SoilsSoil Science Society of America Journal, 1994
- The effect of soil compaction on wheat during early tilleringNew Phytologist, 1990