The detection of specific IgM to infectious bronchitis virus in chicken serum using an elisa
- 3 January 1986
- journal article
- research article
- Published by Informa UK Limited in Avian Pathology
- Vol. 15 (3), 437-446
- https://doi.org/10.1080/03079458608436305
Abstract
An ELISA is reported for the detection of specific IgM to infectious bronchitis virus (IBV) in serum samples from IBV-infected chickens. Both IgM and IgG antibodies to a heterologous, as well as to a homologous, strain could be detected by the ELISA. The IgM response was both rapid and transient, suggesting that its detection could be used as a means of identifying a recent IBV infection. Because IgG appeared to compete successfully with IgM for sites on the viral antigen, it was found necessary to separate IgM from IgG prior to testing in the ELISA.This publication has 7 references indexed in Scilit:
- Problems of Rubella Diagnosis by Various IgM Techniques and the Need for Test CombinationsPublished by Springer Science and Business Media LLC ,1985
- The classification of new serotypes of infectious bronchitis virus isolated from poultry flocks in Britain between 1981 and 1983Avian Pathology, 1984
- Presence of viral antigens and antibody in the trachea of chickens infected with avian infectious bronchitis virusAvian Pathology, 1983
- Comparative studies with an enzyme‐linked immunosorbent assay (Elisa) for antibodies to avian infectious bronchitis virusAvian Pathology, 1981
- The use of chicken tracheal organ cultures for the isolation and assay of avian infectious bronchitis virusArchiv für die gesamte Virusforschung, 1976
- Avian Infectious Bronchitis: Demonstration of Serum IgG- and IgM-Neutralizing Antibody by Sucrose Density-Gradient Centrifugation and Mercaptoethanol ReductionAvian Diseases, 1974