Production and Purification of the Extracellular Ribonuclease of Bacillus Amyloliquefaciens (Barnase) and its Intracellular Inhibitor (Barstar)
- 1 January 1972
- journal article
- research article
- Published by Taylor & Francis Ltd in Preparative Biochemistry
- Vol. 2 (3), 229-242
- https://doi.org/10.1080/00327487208061473
Abstract
Procedures are presented for the large-scale growth of Bacillus amylo-liquefaciens and the concentration and purification of its extracellular ribo-nuclease (barnase). Improvements over earlier methods include economy, the reduction of labor, and Improved purity of product, especially in the elimination of traces of protease. The bacterial cells obtained as a by-product may be used as a starting point for the isolation of barstar, the intracellular inhibitor of barnase1. Experiments relating to the purity, amino-acid composition and molecular weight of the product are also reported.Keywords
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