Sporothrix schenckiicomplex in Iran: Molecular identification and antifungal susceptibility
- 1 March 2016
- journal article
- research article
- Published by Oxford University Press (OUP) in Medical Mycology
- Vol. 54 (6), 593-599
- https://doi.org/10.1093/mmy/myw006
Abstract
Sporotrichosis is a global subcutaneous fungal infection caused by the Sporothrix schenckii complex. Sporotrichosis is an uncommon infection in Iran, and there have been no phenotypic, molecular typing or antifungal susceptibility studies of Sporothrix species. This study aimed to identify nine Iranian isolates of the S. schenckii complex to the species level using colony morphology, carbohydrate assimilation tests, and PCR-sequencing of the calmodulin gene. The antifungal susceptibilities of these Sporothrix isolates to five antifungal agents (amphotericin B (AMB), voriconazole (VRC), itraconazole (ITC), fluconazole (FLC), and terbinafine (TRB)) were also evaluated according to the M27-A3 and M38-A2 protocols of the Clinical and Laboratory Standards Institute for yeast and mycelial phases, respectively. Five of seven clinical isolates were identified as S. schenckii, and two clinical and two environmental isolates were identified as S. globosa. This is the first report of S. globosa in Iran. There was significant agreement (73%) between the results of the phenotypic and genotypic identification methods. TRB and ITC were the most effective antifungals against the Sporothrix isolates. The minimum inhibitory concentration (MIC) values of TRB for the yeast and mycelial phases of S. schenckii differed significantly. There was also a significant difference in the minimum fungicidal concentration (MFC) values of AMB and TRB for the two phases. Considering the low efficacy of VRC and FLC and the wide MIC ranges of AMB (1–16 μg/ml and 1–8 μg/ml for yeast and mycelial forms, respectively) observed in the present study, in vitro antifungal susceptibility testing should be performed to determine appropriate therapeutic regimens.Keywords
This publication has 28 references indexed in Scilit:
- Susceptibility of Sporothrix brasiliensis isolates to amphotericin B, azoles, and terbinafineMedical Mycology, 2014
- Molecular identification of the Sporothrix schenckii complexRevista Iberoamericana de Micologia, 2014
- An environmental Sporothrix as a cause of corneal ulcerMedical Mycology Case Reports, 2013
- Sporothrix schenckii and SporotrichosisClinical Microbiology Reviews, 2011
- MICs and minimum fungicidal concentrations of amphotericin B, itraconazole, posaconazole and terbinafine in Sporothrix schenckiiJournal of Medical Microbiology, 2009
- Sporothrix globosa, a pathogenic fungus with widespread geographical distributionRevista Iberoamericana de Micologia, 2009
- In Vitro Antifungal Susceptibilities of Five Species ofSporothrixAntimicrobial Agents and Chemotherapy, 2008
- Sporothrix luriei: a rare fungus from clinical originMedical Mycology, 2008
- Sporothrix brasiliensis,S. globosa, andS. mexicana, Three NewSporothrixSpecies of Clinical InterestJournal of Clinical Microbiology, 2007
- Genotyping of Sporothrix schenckii by analysis of ribosomal DNA regionsMycoses, 2006