Iron Acquisition from Fe-Pyoverdine by Arabidopsis thaliana
Open Access
- 1 April 2007
- journal article
- Published by Scientific Societies in Molecular Plant-Microbe Interactions®
- Vol. 20 (4), 441-447
- https://doi.org/10.1094/mpmi-20-4-0441
Abstract
Taking into account the strong iron competition in the rhizosphere and the high affinity of pyoverdines for Fe(III), these molecules are expected to interfere with the iron nutrition of plants, as they do with rhizospheric microbes. The impact of Fe-pyoverdine on iron content of Arabidopsis thaliana was compared with that of Fe-EDTA. Iron chelated to pyoverdine was incorporated in a more efficient way than when chelated to EDTA, leading to increased plant growth of the wild type. A transgenic line of A. thaliana overexpressing ferritin showed a higher iron content than the wild type when supplemented with Fe-EDTA but a lower iron content when supplemented with Fe-pyoverdine despite its increased reductase activity, suggesting that this activity was not involved in the iron uptake from pyoverdine. A mutant knockout iron transporter IRT1 showed lower iron and chlorophyll contents when supplemented with Fe-EDTA than the wild type but not when supplemented with Fe-pyoverdine, indicating that, in contrast to iron from EDTA, iron from pyoverdine was not incorporated through the IRT1 transporter. Altogether these data suggest that iron from Fe-pyoverdine was not incorporated in planta through the strategy I, which is based on reductase activity and IRT1 transporter. This is supported by the presence of pyoverdine in planta as shown by enzyme-linked immunosorbent assay and by tracing 15N of 15N-pyoverdine.Keywords
This publication has 38 references indexed in Scilit:
- Iron Transport and Signaling in PlantsAnnual Review of Plant Biology, 2003
- Flux of protons released by wild type and ferritin over-expressor tobacco plants : effect of phosphorus and iron nutritionPlant Physiology and Biochemistry, 2003
- Involvement of Nitrate Reductase and Pyoverdine in Competitiveness of Pseudomonas fluorescens Strain C7R12 in SoilApplied and Environmental Microbiology, 2001
- Hydroxylated Phytosiderophore Species Possess an Enhanced Chelate Stability and Affinity for Iron(III)Plant Physiology, 2000
- Genetic evidence that induction of root Fe(III) chelate reductase activity is necessary for iron uptake under iron deficiency†The Plant Journal, 1996
- Ferric pseudobactin 358 as an iron source for carnationJournal of Plant Nutrition, 1994
- Biological control of fusarium diseases by fluorescent Pseudomonas and non-pathogenic FusariumCrop Protection, 1991
- Formulae for Determination of Chlorophyllous Pigments Extracted with N,N-DimethylformamidePlant Physiology, 1982
- The Fluorescent Pigment of Pseudomonas fluorescens: Biosynthesis, Purification and Physicochemical PropertiesJournal of General Microbiology, 1978
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976