Flavonoids and Phenolic Acids of Sage: Influence of Some Agricultural Factors
- 15 November 2000
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 48 (12), 6081-6084
- https://doi.org/10.1021/jf000440+
Abstract
A reversed-phase high-performance liquid chromatography/diode-array detector procedure is proposed for the determination of six phenolic compounds (caffeic acid, luteolin 7-O-glucoside, rosmarinic acid, apigenin, hispidulin, and cirsimaritin) in sage. The chromatographic separation was achieved using a reversed-phase Spherisorb ODS2 (5-μm particle size, 25.0 × 0.46 cm) column. Of the several extractive solvents assayed only ethyl ether, ethyl acetate, and acetone were able to extract all the compounds mentioned. Best resolution was obtained using a gradient of water/phosphoric acid (999:1) and acetonitrile. Ten samples cultivated in two experimental fields (1997−1999) were analyzed and the individual compounds quantified. Four commercially available samples were also analyzed and the results are discussed. Keywords: Sage (Salvia officinalis); HPLC/DAD; phenolic profile; quality controlKeywords
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