Identification of Specific Transducin α Subunits in Retinal Rod and Cone Photoreceptors
- 3 October 1986
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 234 (4772), 77-80
- https://doi.org/10.1126/science.3529395
Abstract
Transducin is a guanyl nucleotide-binding protein that couples rhodopsin photolysis to hydrolysis of guanosine 3',5'-monophosphate in rod photoreceptor cells of vertebrate retinas. Several complementary DNA clones encoding transducin subunits have recently been characterized. One clone, isolated from a bovine retina complementary DNA library, encodes a previously unidentified polypeptide with an amino acid sequence 78% identical to the sequence of the alpha subunit of bovine rod outer segment transducin. Antibodies to a synthetic peptide with amino acid sequence derived specifically from this novel polypeptide recognize a 41-kilodalton polypeptide in homogenates of bovine retina. Localization of this polypeptide in bovine retina by indirect immunofluorescence demonstrates that it is expressed only in cone outer segments. Antibodies to specific sequences found only in the rod transducin alpha subunit recognize a polypeptide localized only in the rod outer segment. Therefore, bovine rod and cone cells each express structurally related yet significantly different forms of transducin.This publication has 34 references indexed in Scilit:
- Molecular Genetics of Human Color Vision: The Genes Encoding Blue, Green, and Red PigmentsScience, 1986
- Primary structure of the α‐subunit of bovine adenylate cyclase‐inhibiting G‐protein deduced from the cDNA sequenceFEBS Letters, 1986
- Primary structure of the α‐subunit of bovine adenylate cyclase‐stimulating G‐protein deduced from the cDNA sequenceFEBS Letters, 1986
- Light-suppressible, cyclic GMP-sensitive conductance in the plasma membrane of a truncated rod outer segmentNature, 1985
- Cyclic GMP increases photocurrent and light sensitivity of retinal conesNature, 1985
- Cyclic GMP-sensitive conductance in outer segment membrane of catfish conesNature, 1985
- Primary structure of the α-subunit of transducin and its relationship to ras proteinsNature, 1985
- Homologies Between Signal Transducing G Proteins and ras Gene ProductsScience, 1984
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Electrical activity of vertebrate photoreceptorsQuarterly Reviews of Biophysics, 1970