Expression of the human fibroblast interferon gene in Escherichia coli.
- 1 September 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (9), 5230-5233
- https://doi.org/10.1073/pnas.77.9.5230
Abstract
The method of Guarente et al. was used to construct plasmids that direct expression of the human fibroblast interferon (F-IF) gene in E. coli. Two plasmids were recovered. One directs efficient synthesis of a protein whose primary sequence is that of pre-F-IF and the other, that of mature F-IF. Extracts of bacteria synthesizing mature F-IF display antiviral activity characteristic of human F-IF. This activity is lower than that expected from the differential rate of synthesis of the protein. No such activity was detected in extracts of bacteria synthesizing pre-F-IF.Keywords
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