Identification, molecular biotyping and ultrastructural studies of bacterial communities isolated from two damaged frescoes of St Damian's Monastery in Assisi*
- 1 June 2004
- journal article
- Published by Oxford University Press (OUP) in Letters in Applied Microbiology
- Vol. 38 (6), 447-453
- https://doi.org/10.1111/j.1472-765x.2004.01514.x
Abstract
To investigate the composition of the microbial community in biodeterioration of two frescoes in St Damian's Monastery in Assisi.A total of 1292 colonies were isolated from the most deteriorated parts, analysed by microbiological, biomolecular and ultrastructural techniques, and taxonomically classified. Molecular biotyping of Staphylococcus cohnii colonies, one of the most prevalent bacterial species, showed a very restricted genome diversity while Bacillus licheniformis were very homogeneous by RFLP, tDNA-PCR and random-amplified polymorphic DNA. Electron microscopy confirmed heterogeneity of the bacterial population in the different sampling areas.Several of the identified species are widespread in the soil or saprophytes of human skin. Although unable to demonstrate that they are involved in biodeterioration, they may represent trophic elements contributing to fungi-related chromatic alterations when adequate environmental conditions occur. Deterioration may in part be prevented or controlled by adequate air filtering or conditioning of the room.Keywords
This publication has 11 references indexed in Scilit:
- Evaluation of selected features ofStaphylococcus cohnii enabling colonization of humansFolia Microbiologica, 2002
- Distribution of microorganisms on ancient wall paintings as related to associated faunal elementsInternational Biodeterioration & Biodegradation, 2000
- Comparative analyses of the bacterial diversity on two different biodeteriorated wall paintings by DGGE and 16S rDNA sequence analysisInternational Biodeterioration & Biodegradation, 2000
- PCR fingerprinting of whole genomes: the spacers between the 16S and 23S rRNA genes and of intergenic tRNA gene regions reveal a different intraspecific genomic variability of Bacillus cereus and Bacillus licheniformisInternational Journal of Systematic and Evolutionary Microbiology, 1998
- Classification and identification of bacteria: current approaches to an old problem. Overview of methods used in bacterial systematicsJournal of Biotechnology, 1996
- Mycological and ultrastructural studies to evaluate biodeterioration of mural paintings. Detection of fungi and mites in Frescos of the monastery of St Damian in AssisiInternational Biodeterioration & Biodegradation, 1994
- Introduction of biodegradative plasmids to indigenous bacteria from polluted sites in the Czech RepublicInternational Biodeterioration & Biodegradation, 1994
- Characterization of Bacillus licheniformis with the RAPD technique (randomly amplified polymorphic DNA)Letters in Applied Microbiology, 1994
- Microbial communities on damaged frescoes and building materials in the Cathedral of the Nativity of the Virgin in the Pafnutii-Borovskii monastery, RussiaInternational Biodeterioration, 1991
- Genomic fingerprints produced by PCR with consensus tRNA gene primersNucleic Acids Research, 1991