Multicluster nosocomial outbreak of parainfluenza virus type 3 infection in a pediatric oncohematology unit: a phylogenetic study
- 18 April 2009
- journal article
- Published by Ferrata Storti Foundation (Haematologica) in Haematologica
- Vol. 94 (6), 833-839
- https://doi.org/10.3324/haematol.2008.003319
Abstract
Human parainfluenza virus type 3 (hPIV-3) has been reported to cause nosocomial outbreaks of respiratory infection, in particular among hematopoietic stem cell transplantation recipients. From September 2007 through January 2008 several episodes of hPIV-3 infection were observed among young patients followed at the Oncohematology Unit (OHU) or other units of the Pediatrics Department. In 32 young patients (median age 3.5 years, range 21 days-27 years), hPIV-3 infection was diagnosed by direct fluorescent antibody staining of cells from respiratory secretions, and virus quantified by real-time RT-PCR in nasopharyngeal aspirates or bronchoalveolar lavage samples. In addition, the epidemiologic relatedness of hPIV-3 strains was investigated by sequencing two variable regions of the hemagglutinin-neuraminidase gene (nt 1-569 and nt 762-1239). Of the 32 hPIV-3-positive patients, 19 were hematopoietic stem cell transplantation recipients, 8 had hematologic malignancies, and 5 were immunocompetent children. Sixteen patients had upper, and 16 lower respiratory tract infection. All patients but one had high viral load in nasopharyngeal aspirates (>1.0x10(6) RNA copies/mL). One patient died from respiratory failure with a high viral load in bronchoalveolar lavage. Phylogenetic analysis showed that 16/32 strains were identical. Besides this major cluster, three other clusters were identified, each one defining a smaller outbreak. Phylogenetic analysis allows identification of the role of a single or multiple hPIV-3 strains in the person-to-person transmission within an outbreak occurring in clinical units.Keywords
This publication has 16 references indexed in Scilit:
- The human bocavirus role in acute respiratory tract infections of pediatric patients as defined by viral load quantification.2007
- Prolonged outbreak of human parainfluenza virus 3 infection in a stem cell transplant outpatient department: insights from molecular epidemiologic analysisTransplantation and Cellular Therapy, 2004
- Outbreak of Human Parainfluenza Virus 3 Infections in a Hematopoietic Stem Cell Transplant PopulationThe Journal of Infectious Diseases, 2001
- Parainfluenza virus infections after hematopoietic stem cell transplantation: risk factors, response to antiviral therapy, and effect on transplant outcomeBlood, 2001
- Respiratory Disease Due to Parainfluenza Virus in Adult Bone Marrow Transplant RecipientsClinical Infectious Diseases, 1996
- Parainfluenza virus infection in adult bone marrow transplant recipientsEuropean Journal of Clinical Microbiology & Infectious Diseases, 1993
- Molecular Epidemiology of a Parainfluenza Type 3 Virus Outbreak on a Pediatric WardThe Journal of Infectious Diseases, 1993
- Parainfluenza Virus Respiratory Infection after Bone Marrow TransplantationNew England Journal of Medicine, 1992
- Patterns of Shedding of Myxoviruses and Paramyxoviruses in ChildrenThe Journal of Infectious Diseases, 1981
- Persistent parainfluenza virus shedding during isolation at the South PoleNature, 1981