Proinflammatory cytokines expression in noise-induced damaged cochlea
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- 20 January 2006
- journal article
- research article
- Published by Wiley in Journal of Neuroscience Research
- Vol. 83 (4), 575-583
- https://doi.org/10.1002/jnr.20764
Abstract
Recent studies have showed that inflammatory responses occur in inner ear under various damaging conditions including noise-overstimulation. We evaluated the time-dependent expression of proinflammatory cytokines in noise-exposed rat cochlea. Among several detected cytokines, real-time RT-PCR showed that interleukin-1beta (IL-1β) and interleukin-6 (IL-6) were significantly induced 3 hr after noise exposure, and quickly downregulated to the basal level. Tumor necrosis factor-alpha (TNF-α) was also slightly upregulated immediately after noise exposure. Immunohistochemical analysis showed that IL-6 expression was distinctively induced within the lateral side of the spiral ligament. Sequential expression analysis showed that IL-6 immunoreactivity was initially found in the cytoplasm of lateral wall cells, including Type IV and III fibrocytes, and expanded broader throughout the lateral wall, finally to the stria vascularis. Because of the negative Iba-1 staining, IL-6 expression in the early-phase was not due to macrophage or microglia activation. IL-6 was also detected in spiral ganglion neurons at 12 and 24 hr after noise exposure. Our data demonstrates the production of proinflammatory cytokines, including TNF-α, IL-1β, and IL-6, in early phase of noise overstimulated cochlea. IL-6 expression was observed in the spiral ligament, stria vascularis, and spiral ganglion neurons. These cytokines, produced by the cochlear structure itself in response to noise exposure, may initiate an inflammatory response and have some role in the mechanism of noise-induced cochlear damage.Keywords
This publication has 40 references indexed in Scilit:
- Mononuclear phagocytes migrate into the murine cochlea after acoustic traumaJournal of Comparative Neurology, 2005
- Innate Immunity Contributes to Cochlear Adaptive Immune ResponsesAudiology and Neurotology, 2004
- Lateral Wall Histopathology and Endocochlear Potential in the Noise-Damaged Mouse CochleaJournal of the Association for Research in Otolaryngology, 2003
- Quantitative Real-Time RT—PCR Analysis of Inflammatory Gene Expression Associated with Ischemia—Reperfusion Brain InjuryJournal of Cerebral Blood Flow & Metabolism, 2002
- Cytokines and acute neurodegenerationNature Reviews Neuroscience, 2001
- Fibroblasts regulate the switch from acute resolving to chronic persistent inflammationTrends in Immunology, 2001
- Neuronal, astroglial and microglial cytokine expression after an excitotoxic lesion in the immature rat brainEuropean Journal of Neuroscience, 2000
- Expression of IL-6 in the ischemic penumbraNeuroReport, 2000
- Mice Lacking the Basolateral Na-K-2Cl Cotransporter Have Impaired Epithelial Chloride Secretion and Are Profoundly DeafJournal of Biological Chemistry, 1999
- Immunohistochemical Localization of the Na-K-Cl Co-transporter (NKCC1) in the Gerbil Inner EarJournal of Histochemistry & Cytochemistry, 1997