Pat1: a topoisomerase II-associated protein required for faithful chromosome transmission in Saccharomyces cerevisiae
Open Access
- 1 December 1996
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 24 (23), 4791-4797
- https://doi.org/10.1093/nar/24.23.4791
Abstract
Saccharomyces cerevisiae top2 mutants deficient in topoisomerase II activity are defective in chromosome segregation during both mitotic and meiotic cell divisions. To identify proteins that act in concert with topoisomerase II during chromosome segregation in S.cerevisiae, we have used a two-hybrid cloning approach. We report the isolation of the PAT1 gene (for protein associated with topoisomerase II), which encodes a novel 90 kDa proline- and glutamine-rich protein that interacts with a highly conserved, leucine-rich region of topoisomerase II in vivo. Strains lacking Pat1p exhibit a slow growth rate and a phenotype reminiscent of conditional top2 mutants grown at the semi-permissive temperature; most notably, a reduced fidelity of chromosome segregation during both mitosis and meiosis. These findings indicate that the PAT1 gene is necessary for accurate chromosome transmission during cell division in eukaryotic cells and suggest that the interaction of Pat1p and topoisomerase II is an important component of this function.Keywords
This publication has 26 references indexed in Scilit:
- Identification and initial characterization of the cytosolic protein Ycr77pYeast, 1995
- A novel genetic system to detect protein–protein interactionsNature, 1989
- A subthreshold level of DNA topoisomerases leads to the excision of yeast rDNA as extrachromosomal ringsCell, 1989
- DNA topoisomerase II must act at mitosis to prevent nondisjunction and chromosome breakage.Molecular and Cellular Biology, 1989
- Mitotic recombination in the rDNA of S. cerevisiae is suppressed by the combined action of DNA topoisomerases I and IICell, 1988
- DNA topoisomerase II is required for condensation and separation of mitotic chromosomes in S. pombeCell, 1987
- Chromosome Segregation in Mitosis and MeiosisAnnual Review of Cell Biology, 1985
- DNA TOPOISOMERASESAnnual Review of Biochemistry, 1985
- DNA topoisomerase II is required at the time of mitosis in yeastCell, 1985
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970