Myocyte hypertrophy in neonatal rat heart cultures and its regulation by serum and by catecholamines.
- 1 December 1982
- journal article
- abstracts
- Published by Ovid Technologies (Wolters Kluwer Health) in Circulation Research
- Vol. 51 (6), 787-801
- https://doi.org/10.1161/01.res.51.6.787
Abstract
The role of hormones and other humoral factors in the regulation of myocardial hypertrophy has been difficult to evaluate. We asked whether myocardial cell hypertrophy could be demonstrated in cultures from the day-old rat ventricle and evaluated the effect of serum concentration and catecholamines on the growth process. Two single-cell preparations were used: serum-supplemented, bromodeoxyuridine-treated cultures and serum-free cultures with transferrin and insulin. Both preparations were characterized by myocardial cell predominance (about 75--80% of total cells) and constant cell numbers. Myocardial cell size was documented by photomicroscopy and quantified by volume (microscopic diameter of suspended cells), surface area (planimetry of attached cells), and total cell protein concentration (Lowry method and cell counts). Growth was also evaluated in pure nonmyocardial cell cultures. In cultures with 5% (vol/vol) serum, myocardial cell size increased 2- to 3-fold over 11 days in culture. Final volume, surface area, and protein concentration were about 3000 micrometer3/cell, 5000 micrometer2/cell, and 1500 pg/cell, respectively. Serum had a dose-related effect on myocardial cell hypertrophy; myocardial cell size increased about 4-fold when serum concentration was increased from 0% to 5% or 10%. Cells maintained in serum-free medium with transferrin and insulin (each 10 microgram/ml) did not hypertrophy, but did remain responsive to the growth-promoting activity of serum. Chronic exposure to isoproterenol or norepinephrine (1 microM) significantly stimulated myocardial cell hypertrophy. This stimulation was dose-related, was not blocked by equimolar propranolol, was not associated with a sustained chronotropic effect, and was more pronounced in the serum-free preparation. In pure cultures of nonproliferating (bromodeoxyuridine-treated) nonmyocardial cells, cell size also increased with time in culture, but variation in serum concentration and addition of norepinephrine had no significant effect on cell size. Myocardial cell hypertrophy occurs in culture and is regulated by variations in the culture medium, including serum, with its contained hormones and growth factors, and catecholamines. The culture preparation can be used to explore the regulation of myocardial cell hypertrophy by nonhemodynamic factors.Keywords
This publication has 16 references indexed in Scilit:
- Effect of high and low density lipoproteins on proliferation of cultured bovine vascular endothelial cells.JCI Insight, 1980
- Physiology and Pathophysiology of the Human Sympathoadrenal Neuroendocrine SystemThe New England Journal of Medicine, 1980
- Surface morphology and cell size measurement of isolated rat cardiac myocytes*1Journal of Molecular and Cellular Cardiology, 1979
- Metabolic and cardiovascular responses to prolonged noradrenaline load and their antagonism by beta blockade in the ratActa Physiologica Scandinavica, 1978
- Role of cell shape in growth controlNature, 1978
- Protein metabolism during growth of vero cellsJournal of Cellular Physiology, 1977
- Norepinephrine—The myocardial hypertrophy hormone?American Heart Journal, 1976
- Measurement of the rate and velocity of movement by single heart cells in cultureThe American Journal of Cardiology, 1973
- Catecholamine and Dibutyryl Cyclic AMP Effects on Myosin Adenosine Triphosphatase in Cultured Rat Heart CellsScience, 1973
- Studies on isoproterenol-induced cardiomegaly in ratsAmerican Heart Journal, 1969