In Vivo Bone Formation by Human Bone Marrow Stromal Cells: Reconstruction of the Mouse Calvarium and Mandible
- 1 September 2006
- journal article
- Published by Oxford University Press (OUP) in The International Journal of Cell Cloning
- Vol. 24 (9), 2140-2149
- https://doi.org/10.1634/stemcells.2005-0567
Abstract
Bone marrow stromal cells (BMSCs) contain a subset of multipotent cells with the potential to repair hard-tissue defects. Mouse BMSCs, combined with a collagen carrier, can close critical-sized homologous mouse calvarial defects, but this new bone has a poor union with the adjacent calvarium. When human BMSCs are transplanted for the purpose of engineering new bone, best results can be achieved if the cells are combined with hydroxyapatite/tricalcium phosphate (HA/TCP) particles. Here, we demonstrate that transplantation of cultured human BMSCs in conjunction with HA/TCP particles can be used successfully to close mouse craniofacial bone defects and that removal of the periosteum from the calvarium significantly enhances union with the transplant. Transplants were followed for up to 96 weeks and were found to change in morphology but not bone content after 8 weeks; this constitutes the first description of human BMSCs placed long-term to heal bone defects. New bone formation continued to occur in the oldest transplants, confirmed by tetracycline labeling. Additionally, the elastic modulus of this engineered bone resembled that of the normal mouse calvarium, and our use of atomic force microscopy (AFM)-based nanoindentation offered us the first opportunity to compare these small transplants against equally minute mouse bones. Our results provide insights into the long-term behavior of newly engineered orthotopic bone from human cells and have powerful implications for therapeutic human BMSC transplantation.Keywords
This publication has 31 references indexed in Scilit:
- In vivo bone formation by human marrow stromal cells in biodegradable scaffolds that release dexamethasone and ascorbate-2-phosphateBiochemical and Biophysical Research Communications, 2005
- Extensive augmentation of the alveolar ridge using autogenous calvarial split bone grafts for dental rehabilitationClinical Oral Implants Research, 2004
- Bone Tissue-Engineered Implants Using Human Bone Marrow Stromal Cells: Effect of Culture Conditions and Donor AgeTissue Engineering, 2002
- Number and Proliferative Capacity of Osteogenic Stem Cells Are Maintained During Aging and in Patients with OsteoporosisJournal of Bone and Mineral Research, 2001
- Exogenously Regulated Stem Cell-Mediated Gene Therapy for Bone RegenerationMolecular Therapy, 2001
- Effect of age and sampling site on the chondro‐osteogenic potential of rabbit marrow‐derived mesenchymal progenitor cellsJournal of Orthopaedic Research, 2000
- Age-Related Osteogenic Potential of Mesenchymal Stromal Stem Cells from Human Vertebral Bone MarrowJournal of Bone and Mineral Research, 1999
- Single-Colony Derived Strains of Human Marrow Stromal Fibroblasts Form Bone After Transplantation In VivoJournal of Bone and Mineral Research, 1997
- A method for interpreting the data from depth-sensing indentation instrumentsJournal of Materials Research, 1986
- Cranioplasty and the Post-Traumatic SyndromeJournal of Neurosurgery, 1948