Molecular cartography of the human skin surface in 3D
- 30 March 2015
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 112 (17), E2120-E2129
- https://doi.org/10.1073/pnas.1424409112
Abstract
The human skin is an organ with a surface area of 1.5–2 m2 that provides our interface with the environment. The molecular composition of this organ is derived from host cells, microbiota, and external molecules. The chemical makeup of the skin surface is largely undefined. Here we advance the technologies needed to explore the topographical distribution of skin molecules, using 3D mapping of mass spectrometry data and microbial 16S rRNA amplicon sequences. Our 3D maps reveal that the molecular composition of skin has diverse distributions and that the composition is defined not only by skin cells and microbes but also by our daily routines, including the application of hygiene products. The technological development of these maps lays a foundation for studying the spatial relationships of human skin with hygiene, the microbiota, and environment, with potential for developing predictive models of skin phenotypes tailored to individual health.Keywords
This publication has 48 references indexed in Scilit:
- MS/MS networking guided analysis of molecule and gene cluster familiesProceedings of the National Academy of Sciences, 2013
- Structure, function and diversity of the healthy human microbiomeNature, 2012
- Mass spectral molecular networking of living microbial coloniesProceedings of the National Academy of Sciences, 2012
- An improved Greengenes taxonomy with explicit ranks for ecological and evolutionary analyses of bacteria and archaeaThe ISME Journal, 2011
- Spectral archives: extending spectral libraries to analyze both identified and unidentified spectraNature Methods, 2011
- Skin microbiota: Microbial community structure and its potential association with health and diseaseInfection, Genetics and Evolution, 2011
- The skin microbiomeNature Reviews Microbiology, 2011
- QIIME allows analysis of high-throughput community sequencing dataNature Methods, 2010
- Bacterial diversity in the oral cavity of 10 healthy individualsThe ISME Journal, 2010
- The Human Microbiome ProjectNature, 2007