Heterogeneous phenotypes of expression of the NKB1 natural killer cell class I receptor among individuals of different human histocompatibility leukocyte antigens types appear genetically regulated, but not linked to major histocompatibililty complex haplotype.
Open Access
- 1 April 1996
- journal article
- research article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 183 (4), 1817-1827
- https://doi.org/10.1084/jem.183.4.1817
Abstract
Natural killer (NK) cells that express the NKB1 receptor are inhibited from killing target cells that possess human histocompatibility leukocyte antigen (HLA) B molecules bearing the Bw4 serological epitope. To investigate whether NKB1 expression is affected by HLA type, peripheral blood lymphocytes of 203 HLA-typed donors were examined. Most donors had a single population of NKB1+ cells, but some had two populations expressing different cell surface levels of NKB1, and others had no detectable NKB1+ cells. Among the donors expressing NKB1, both the relative abundance of NKB1+ NK cells and their level of cell surface expression varied substantially. The percentage of NKB1+ NK cells ranged from 0 to >75% (mean 14.7%), and the mean fluorescence of the positive population varied over three orders of magnitude. For each donor, the small percentage of T cells expressing NKB1 (usually <2%), had a pattern of expression mirroring that of the NK cells. NKB1 expression by NK and T cells remained stable over the 2-yr period that five donors were tested. Patterns of NKB1 expression were not associated with Bw4 or Bw6 serotype of the donor or with the presence of any individual HLA-A or -B antigens. Cells expressing NKB1 are often found in donors who do not possess an appropriate class I ligand, and can be absent in those who express Bw4+ HLA-B antigens. Family studies further suggested that the phenotype of NKB1 expression is inherited but not HLA linked. Whereas identical twins show matching patterns of NKB1 expression, HLA-identical siblings can differ in NKB1 expression, and conversely, HLA-disparate siblings can be similar. Thus NKB1 expression phenotypes are tightly regulated and extremely heterogeneous, but not correlated with HLA type.This publication has 27 references indexed in Scilit:
- Killer cell inhibitory receptors specific for HLA-C and HLA-B identified by direct binding and by functional transferImmunity, 1995
- MHC class I alloantigen specificity of Ly-49+ IL-2-activated natural killer cellsNature, 1992
- In search of the ‘missing self’: MHC molecules and NK cell recognitionImmunology Today, 1990
- Reversal of natural killing susceptibility in target cells expressing transfected class I HLA genes.Proceedings of the National Academy of Sciences of the United States of America, 1989
- Demonstration by class I gene transfer that reduced susceptibility of human cells to natural killer cell‐mediated lysis is inversely correlated with HLA class I antigen expressionEuropean Journal of Immunology, 1989
- Biology of Natural Killer CellsAdvances in Immunology, 1989
- Transfer and expression of three cloned human non-HLA-A,B,C class I major histocompatibility complex genes in mutant lymphoblastoid cells.Proceedings of the National Academy of Sciences of the United States of America, 1988
- The primary structure of HLA-A32 suggests a region involved in formation of the Bw4/Bw6 epitopes.1986
- Selective rejection of H–2-deficient lymphoma variants suggests alternative immune defence strategyNature, 1986
- Subpopulations of human natural killer cells defined by expression of the Leu-7 (HNK-1) and Leu-11 (NK-15) antigens.The Journal of Immunology, 1983