Advances in Swine In Vitro Embryo Production Technologies
- 17 May 2010
- journal article
- review article
- Published by Wiley in Reproduction in Domestic Animals
- Vol. 45 (s2), 40-48
- https://doi.org/10.1111/j.1439-0531.2010.01623.x
Abstract
Recent advances in new technologies to produce cloned and genetically modified pigs involve manipulating oocytes and/or embryos in vitro. Although a great deal of progress has been made, the current IVM-IVF systems still result in major problems: a high rate of polyspermy; and a low development rate and low quality of blastocysts for in vitro compared with the in vivo-produced embryos. This study summarizes recent advancements in IVM-IVF-IVC porcine systems. Recent methods to select monospermic embryos are also discussed. Finally, achievements in vitrification and in somatic cell nuclear transfer are discussed.Keywords
This publication has 107 references indexed in Scilit:
- 335 EFFECT OF MEM VITAMINS AND FORSKOLIN ON IN VITRO EMBRYO PRODUCTION AND SOPS-VITRIFICATION ABILITY OF IN VITRO DERIVED PORCINE BLASTOCYSTSReproduction, Fertility and Development, 2010
- Centrosome abnormalities during porcine oocyte agingEnvironmental and Molecular Mutagenesis, 2009
- In VitroFertilization (IVF) in Straws and a Short Gamete Coincubation Time Improves the Efficiency of Porcine IVFReproduction in Domestic Animals, 2008
- A Comparative Study on the Efficiency of Two Enucleation Methods in Pig Somatic Cell Nuclear Transfer: Effects of the Squeezing and the Aspiration MethodsAnimal Biotechnology, 2008
- Reduced Polyspermic Penetration in Porcine Oocytes Inseminated in a New In Vitro Fertilization (IVF) System: Straw IVF1Biology of Reproduction, 2003
- Effect of the volume of medium and number of oocytes during in vitro fertilization on embryo development in pigsTheriogenology, 2003
- Nuclear Cloning and Epigenetic Reprogramming of the GenomeScience, 2001
- Substrate utilization in porcine embryos cultured in NCSU23 and G1.2/G2.2 sequential culture mediaMolecular Reproduction and Development, 2001
- Sperm Selection by a Climbing-over-a-Wall IVF Method Reduces the Incidence of Polyspermic Penetration of Porcine Oocytes.The Journal of Reproduction and Development, 2000
- Ice-free cryopreservation of mouse embryos at −196 °C by vitrificationNature, 1985