Starch-Branching Enzymes Preferentially Associated with A-Type Starch Granules in Wheat Endosperm
- 1 September 2000
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 124 (1), 265-272
- https://doi.org/10.1104/pp.124.1.265
Abstract
Two starch granule-bound proteins (SGP), SGP-140 and SGP-145, were preferentially associated with A-type starch granules (>10 μm) in developing and mature wheat (Triticum aestivum) kernels. Immunoblotting and N-terminal sequencing suggested that the two proteins were different variants of SBEIc, a 152-kD isoform of wheat starch-branching enzyme. Both SGP-140 and SGP-145 were localized to the endosperm starch granules but were not found in the endosperm soluble fraction or pericarp starch granules younger than 15 d post anthesis (DPA). Small-size starch granules (10 μm). In contrast, small-size starch granules harvested after 15 DPA contained only low amounts of SGP-140 and SGP-145 and developed mainly into B-type starch granules (<10 μm). Polypeptides of similar mass and immunologically related to SGP-140 and/or SGP-145 were also preferentially incorporated into A-type starch granules of barley (Hordeum vulgare), rye (Secale cereale), and triticale (× Triticosecale Wittmack) endosperm, which like wheat endosperm have a bimodal starch granule size distribution.Keywords
This publication has 26 references indexed in Scilit:
- Isolation of a cDNA Encoding a Granule-Bound 152-Kilodalton Starch-Branching Enzyme in WheatPlant Physiology, 2000
- Differential Expression and Properties of Starch Branching Enzyme Isoforms in Developing Wheat EndospermPlant Physiology, 1997
- From Glycogen to Amylopectin: A Model for the Biogenesis of the Plant Starch GranuleCell, 1996
- Physical Association of Starch Biosynthetic Enzymes with Starch Granules of Maize Endosperm (Granule-Associated Forms of Starch Synthase I and Starch Branching Enzyme II)Plant Physiology, 1996
- Metabolism of starch synthesis in developing grains of the shx shrunken mutant of barley (Hordeum vulgare )Physiologia Plantarum, 1995
- An analysis of soluble starch synthase isozymes from the developing grains of normal and shx cv. Bomi barley (Hordeum vulgare)Physiologia Plantarum, 1993
- Soluble isoforms of starch synthase and starch‐branching enzyme also occur within starch granules in developing pea embryosThe Plant Journal, 1993
- A novel shrunken endosperm mutant of barleyPhysiologia Plantarum, 1990
- The Size Distribution Among Starch Granules in Wheat EndospermStarch ‐ Stärke, 1973
- Starch Synthesis Studies in Zea maysPlant Physiology, 1970