Identification of the biliary metabolites of (±)-3-dimethylamino-1,1-diphenylbutane HC1 (recipavrin) in rats
- 1 January 1990
- journal article
- research article
- Published by Taylor & Francis Ltd in Xenobiotica
- Vol. 20 (10), 999-1024
- https://doi.org/10.3109/00498259009046822
Abstract
1. The in vivo biliary metabolites of (±)-3-dimethylamino-1, 1-diphenylbutane hydrochloride (recipavrin) isolated from Wistar rats have been characterized by g.l.c.-mass spectrometry. 2. Non-conjugated metabolites include recipavrin (1), norrecipavrin (2), diphenylbutanone (3), diphenylbutanone oxime (4), diphenylbutanone phenol (12), diphenylbutanone oxime phenol (14), recipavrin phenol (19), diphenyibutanone O-methylcatechol (16) and diphenylbutanone oxime O-methylcatechol (18). 3. Following β-glucuronidase hydrolysis and extraction from pH10 solution, diphenylbutanone (3), diphenylbutanone oxime (4), an unidentified compound (6), primary amine (8), norrecipavrin (2), recipavrin (1), phenols (12, 14, 15), norrecipavrin phenol (13), O-methylcatechols (16, 18), diphenylbutanol O-methylcatechol (17), recipavrin O-methylcatechol (19) and a secondary formamide (5) were identified by g.l.c.-mass spectrometry. 4. Various extraction solvents were employed in sample workup. The formamide (5) was present regardless of solvent used, while the trace presence of secondary acetamide (7) may be associated with the use of ethyl acetate. 5. Metabolites isolated after β-glucuronidase hydrolysis were characterized by g.l.c.-mass spectrometry of the underivatized form, and as the trimethylsilyl (TMS) derivatives, or following methylation with diazomethane or trimethylanilinium hydroxide (TMAH).Keywords
This publication has 38 references indexed in Scilit:
- Characterization of new oxidation products of the perchlorate salt of the methadone metabolite,(±)-2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP)Tetrahedron, 1986
- Methadone metabolism in the ratin vivo: identification of a novel formamide metaboliteXenobiotica, 1985
- Identification of fendiline metabolites in human urineJournal of Mass Spectrometry, 1983
- Disappearing N-hydroxy compoundsJournal of Pharmacy and Pharmacology, 1979
- The Metabolism of Amphetamines in MammalsDrug Metabolism Reviews, 1976
- The metabolism of (+)- and (-)-amphetamine and (+)- and (-)-dimethylamphetamine in rabbits in vivoBiochemical Journal, 1972
- Biliary excretion of amphetamine and methamphetamine in the ratBiochemical Journal, 1972
- Metabolic Oxidation of Aliphatic Basic Nitrogen Atoms and their α-Carbon AtomsXenobiotica, 1971
- The biochemistry of aromatic amines. 2-Formamido-1-naphthyl hydrogen sulphate, a metabolite of 2-naphthylamineBiochemical Journal, 1966
- The Leuckart Reaction with β-Phenyl KetonesJournal of the American Chemical Society, 1951