Cholinergic innervation of the cat striate cortex: A choline acetyltransferase immunocytochemical analysis

Abstract

In area 17 of adult cats the morphology, distribution, and synaptology of cholinergic elements were examined by immunocytochemical methods with a monoclonal antibody against choline acetyltransferase (ChAT). ChAT(+) fibers are present throughout the entire depth of the cortex but are particularly dense in layer I. Typically these fibers are very thin and possess numerous irregularly spaced varicosities. Except in layer I and deep layer VI, where the fibers tend to run parallel to the pial surface, they appear to be randomly oriented. At the electron microscope level, immunolabeling was present in unmyelinated fibers of irregular contour and diameter. Most of the ChAT(+) varicose profiles contained mitochondria and round vesicles; Synaptic complexes were relatively infrequent and tended to be of the symmetrical type. They were located mostly on dendritic shafts and only rarely on cell bodies and dendritic spines. Both pyramidal and nonpyramidal cells were found to be innervated by cholinergic afferents. These anatomical data are consistent with the known physiology of acetylcholine in the visual cortex, which indicates that it acts as a modulator of cortical excitability.