Lactic acid bacteria associated with vacuum-packed cooked meat product spoilage: population analysis by rDNA-based methods
Open Access
- 31 July 2006
- journal article
- Published by Oxford University Press (OUP) in Journal of Applied Microbiology
- Vol. 102 (2), 498-508
- https://doi.org/10.1111/j.1365-2672.2006.03081.x
Abstract
Aim: To determine the lactic acid bacteria (LAB) implicated in bloating spoilage of vacuum-packed and refrigerated meat products. Methods and Results: A total of 18 samples corresponding to four types of meat products, with and without spoilage symptoms, were studied. In all, 387 colonies growing on de Man, Rogosa and Sharpe, yeast glucose lactose peptone and trypticase soy yeast extract plates were identified by internal spacer region (ISR), ISR-restriction fragment length polymorphism and rapid amplified ribosomal DNA restriction analysis profiles as Lactobacillus (37%), Leuconostoc (43%), Carnobacterium (11%), Enterococcus (4%) and Lactococcus (2%). Leuconostoc mesenteroides dominated the microbial population of spoiled products and was always present at the moment bloating occurred. Lactobacillus sakei, Lactobacillus plantarum and Lactobacillus curvatus were found in decreasing order of abundance. The analysis of two meat products, ‘morcilla’ and ‘fiambre de magro adobado’ obtained from production lines revealed a common succession pattern in LAB populations in both products and showed that Leuc. mesenteroides became the main species during storage, despite being below the detection level of culture methods after packing. Conclusions: Our results pointed to Leuc. mesenteroides as the main species responsible for bloating spoilage in vacuum-packed meat products. Significance and Impact of the Study: Prevention of bloating spoilage in vacuum-packed cooked meat products requires the sensitive detection of Leuc. mesenteroides (i.e. by PCR).Keywords
This publication has 30 references indexed in Scilit:
- Identification of lactic acid bacteria: culture-dependent and culture-independent methodsTrends in Food Science & Technology, 2004
- Behavior of Psychrotrophic Lactic Acid Bacteria Isolated from Spoiling Cooked Meat ProductsApplied and Environmental Microbiology, 2003
- Phenotypic and phylogenetic characterization of a novel Lactobacillus species from human sources: description of Lactobacillus iners sp. nov.International Journal of Systematic and Evolutionary Microbiology, 1999
- Rapid species identification within two groups of closely related lactobacilli using PCR primers that target the 16S/23S rRNA spacer regionFEMS Microbiology Letters, 1998
- Characterization of Lactobacillus sake strains associating with production of ropy slime by randomly amplified polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE) patternsInternational Journal of Food Microbiology, 1996
- rRNA gene restriction patterns as a characterization tool for Lactobacillus sake strains producing ropy slimeInternational Journal of Food Microbiology, 1996
- The relationship between the phenotypic properties of bacteria from chill‐stored meat and spoilage processesJournal of Applied Bacteriology, 1992
- Quantification and characterization of microbial populations associated with spoiled, vacuum-packed Vienna sausagesFood Microbiology, 1991
- Rapid extraction of bacterial genomic DNA with guanidium thiocyanateLetters in Applied Microbiology, 1989
- A MEDIUM FOR THE CULTIVATION OF LACTOBACILLIJournal of Applied Bacteriology, 1960