Hydrophilic interaction chromatography coupled to MS for metabonomic/metabolomic studies
- 1 March 2010
- journal article
- review article
- Published by Wiley in Journal of Separation Science
- Vol. 33 (6-7), 716-727
- https://doi.org/10.1002/jssc.200900803
Abstract
Hydrophilic interaction chromatography (HILIC) is a relatively recently introduced mode of liquid‐phase separations. Recently, HILIC has been used for coupling to MS in metabonomic/metabolomic studies to provide a complementary tool to the widely used reversed‐phase (RP) chromatographic separations. The combination of HILIC with MS detection covers a number of polar metabolites that are typically nonretained in RPLC‐mode separations and thus enlarging the number of detected analytes. This way of metabolite profiling thus provides more comprehensive metabolome coverage than using RP chromatography alone. This review describes the applications and the utility of HILIC‐MS in metabolomic/metabonomic studies and highlights certain characteristic examples in the life and plant‐food sciences.Keywords
This publication has 41 references indexed in Scilit:
- MetabonomicsNature, 2008
- Analytical Strategies in MetabonomicsJournal of Proteome Research, 2006
- Mass spectrometry‐based metabolomicsMass Spectrometry Reviews, 2006
- HPLC-MS-based methods for the study of metabonomicsJournal of Chromatography B, 2005
- Metabonomics technologies and their applications in physiological monitoring, drug safety assessment and disease diagnosisBiomarkers, 2004
- Metabonomics: a platform for studying drug toxicity and gene functionNature Reviews Drug Discovery, 2002
- Metabolomics – the link between genotypes and phenotypesPlant Molecular Biology, 2002
- Metabolite profiling for plant functional genomicsNature Biotechnology, 2000
- 'Metabonomics': understanding the metabolic responses of living systems to pathophysiological stimuli via multivariate statistical analysis of biological NMR spectroscopic dataXenobiotica, 1999
- High resolution proton magnetic resonance spectroscopy of biological fluidsProgress in Nuclear Magnetic Resonance Spectroscopy, 1989