Glyceraldehyde-3-Phosphate Dehydrogenase Varies With Age in Glycolytic Muscles of Rats
Open Access
- 1 March 2000
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journals of Gerontology, Series A: Biological Sciences and Medical Sciences
- Vol. 55 (3), B160-B164
- https://doi.org/10.1093/gerona/55.3.b160
Abstract
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA, protein, and enzyme activity levels in hindlimb muscles of adult and senescent Fischer 344 × Brown Norway rats were investigated. Soleus muscles from adult and senescent rats had similar levels of GAPDH. In contrast, muscles containing a large proportion of glycolytic fibers had lower GAPDH levels in senescent rats relative to these muscles in adult rats; this was observed at both the mRNA and protein levels. These data indicate that skeletal muscle glycolytic capacity of fast muscles is diminished with age and that it may be caused by changes at the level of transcription. Also, because GAPDH mRNA levels change with age in several rat muscles, GAPDH mRNA is not always a proper internal control for mRNA analyses of aging skeletal muscle.Keywords
This publication has 11 references indexed in Scilit:
- Myogenin, MyoD, and myosin expression after pharmacologically and surgically induced hypertrophyJournal of Applied Physiology, 1998
- Diversity of ACTH (cAMP)‐dependent transcription of bovine steroid hydroxylase genesThe FASEB Journal, 1997
- Pathologic Characterization of Brown Norway, Brown Norway x Fischer 344, and Fischer 344 x Brown Norway Rats With Relation to AgeThe Journals of Gerontology, Series A: Biological Sciences and Medical Sciences, 1996
- Enhanced Expression of Myogenic Regulatory Genes in Aging Skeletal MuscleExperimental Cell Research, 1995
- Effects of ageing on the motor unitProgress in Neurobiology, 1995
- Anaerobic energy provision in aged skeletal muscle during tetanic stimulationJournal of Applied Physiology, 1991
- Morphological and biochemical changes in old rat muscles: effect of increased useJournal of Applied Physiology, 1989
- Regulation of skeletal muscle 6-phosphofructo-1-kinase during aging and developmentMechanisms of Ageing and Development, 1986
- Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: genomic complexity and molecular evolution of the geneNucleic Acids Research, 1985
- Muscle fiber type composition of the rat hindlimbJournal of Anatomy, 1984