Generation of conditional Hoxc8 loss‐of‐function and Hoxc8→Hoxc9 replacement alleles in mice
- 20 July 2009
- Vol. 47 (10), 680-687
- https://doi.org/10.1002/dvg.20547
Abstract
The Hox family of transcription factors are expressed at different domains along the rostrocaudal (R‐C) body axis during development. To examine the function of Hoxc8 and Hoxc9 in specific cell types and at different developmental times, we have generated and characterized loxP flanked (floxed) Hoxc8 and Hoxc8→Hoxc9 replacement alleles of mice, with either GFP or LacZ reporters. Although all four alleles of mice behave like wild‐type controls in motor behavioral testing, slight differences in endogenous Hox gene expression were observed among these alleles depending on the type of reporters used and the presence of Hoxc9 cDNA in the targeting constructs. The efficiency of Cre‐mediated recombination was evaluated by crossing these mice with the Nestin‐cre and Isl1‐cre mice, and the loss of Hoxc8 expression with or without Hoxc9 misexpression was confirmed in embryonic spinal cord. In addition, an upregulation of reporter gene expression was observed after Cre‐mediated recombination. These mice will be useful tools to analyze Hox gene function in a cell type‐specific manner. genesis 47:680–687, 2009.Keywords
This publication has 22 references indexed in Scilit:
- Hoxc10 and Hoxd10 regulate mouse columnar, divisional and motor pool identity of lumbar motoneuronsDevelopment, 2008
- The function of growth/differentiation factor 11 (Gdf11) in rostrocaudal patterning of the developing spinal cordDevelopment, 2006
- A Hox Regulatory Network Establishes Motor Neuron Pool Identity and Target-Muscle ConnectivityCell, 2005
- Retinaldehyde dehydrogenase 2 and Hoxc8 are required in the murine brachial spinal cord for the specification of Lim1+ motoneurons and the correct distribution of Islet1+ motoneuronsDevelopment, 2005
- MicroRNA-Directed Cleavage of HOXB8 mRNAScience, 2004
- Hoxa10 andHoxd10 coordinately regulate lumbar motor neuron patterningJournal of Neurobiology, 2003
- Assigning the Positional Identity of Spinal Motor NeuronsNeuron, 2001
- Visualizing an Olfactory Sensory MapCell, 1996
- Hox Genes and Regionalization of the Nervous SystemAnnual Review of Neuroscience, 1994
- Differential staining of cartilage and bone in whole mouse fetuses by alcian blue and alizarin red STeratology, 1980