aubergine mutations in Drosophila melanogaster impair P cytotype determination by telomeric P elements inserted in heterochromatin.
- 14 September 2004
- journal article
- Published by Springer Science and Business Media LLC in Molecular Genetics and Genomics
- Vol. 272 (3), 336-343
- https://doi.org/10.1007/s00438-004-1061-1
Abstract
Transposable P elements inserted in the heterochromatic Telomeric Associated Sequences on the X chromosome (1A site) of Drosophila melanogaster have a very strong capacity to elicit the P cytotype, a maternally transmitted condition which represses P element transposition and P-induced hybrid dysgenesis. This repressive capacity has previously been shown to be sensitive to mutant alleles of the gene Su(var)205, which encodes HP1 (Heterochromatin Protein 1), thus suggesting a role for chromatin structure in repression. Since an interaction between heterochromatin formation and RNA interference has been reported in various organisms, we tested the effect of mutant alleles of aubergine, a gene that has been shown to play a role in RNA interference in Drosophila, on the repressive properties of telomeric P elements. Seven out of the eight mutant alleles tested clearly impaired the repressive capacities of the two independent telomeric P insertions at 1A analyzed. P repression by P strains whose repressive capacities are not linked to the presence of P copies at 1A were previously found to be insensitive to Su(var)205; here, we show that they are also insensitive to aubergine mutations. These results strongly suggest that both RNA interference and heterochromatin structure are involved in the establishment of the P cytotype elicited by telomeric P elements, and reinforce the hypothesis that different mechanisms for repression of P elements exist which depend on the chromosomal location of the regulatory copies of P.Keywords
This publication has 40 references indexed in Scilit:
- The BDGP Gene Disruption ProjectGenetics, 2004
- Transposon silencing in the Caenorhabditis elegans germ line by natural RNAiNature, 2003
- ARGONAUTE4 Control of Locus-Specific siRNA Accumulation and DNA and Histone MethylationScience, 2003
- Regulation of Heterochromatic Silencing and Histone H3 Lysine-9 Methylation by RNAiScience, 2002
- RNAi is activated during Drosophila oocyte maturation in a manner dependent on aubergine and spindle-EGenes & Development, 2002
- P-element repressor autoregulation involves germ-line transcriptional repression and reduction of third intron splicing.Genes & Development, 1995
- Mutability, sterility and suppression in P—M hybrid dysgenesis: the influence of P subline, cross, chromosome, sex and P-element structureGenetics Research, 1993
- Distribution and structure of cloned P elements from theDrosophila melanogasterP strain π2Genetics Research, 1992
- Localization of P elements, copy number regulation, and cytotype determination inDrosophila melanogasterGenetics Research, 1990
- Hybrid dysgenesis inDrosophila melanogaster: rules of inheritance of female sterilityGenetics Research, 1979