Interaction of Enteric Bacterial Pathogens with Murine Embryonic Stem Cells
Open Access
- 1 February 2009
- journal article
- research article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 77 (2), 585-597
- https://doi.org/10.1128/iai.01003-08
Abstract
Embryonic stem (ES) cells are susceptible to genetic manipulation and retain the potential to differentiate into diverse cell types, which are factors that make them potentially attractive cells for studying host-pathogen interactions. Murine ES cells were found to be susceptible to invasion bySalmonella entericaserovar Typhimurium andShigella flexneriand to the formation of attaching and effacing lesions by enteropathogenicEscherichia coli. S. entericaserovar Typhimurium andS. flexnericell entry was dependent on theSalmonellapathogenicity island 1 andShigella mxi/spatype III secretion systems, respectively. Microscopy studies indicated that bothS. entericaserovar Typhimurium andS. flexneriwere located in intracellular niches in ES cells that were similar to the niches occupied in differentiated cells. ES cells were eventually killed following bacterial invasion, but no evidence of activation of classical caspase-associated apoptotic or innate immune pathways was found. To demonstrate the potential of mutant ES cells, we employed an ES cell line defective in cholesterol synthesis and found that the mutant cells were less susceptible to infection bySalmonellaandShigellathan the parental ES cells. Thus, we highlighted the practical use of genetically modified ES cells for studying microbe-host interactions.Keywords
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