Structure of the PRYSPRY‐domain: Implications for autoinflammatory diseases

Abstract

We determined the first structure of PRYSPRY, a domain found in over 500 different proteins, involved in innate immune signaling, cytokine signaling suppression, development, cell growth and retroviral restriction. The fold encompasses a 7‐stranded and a 6‐stranded antiparallel β‐sheet, arranged in a β‐sandwich. In the crystal, PRYSPRY forms a dimer where the C‐terminus of an acceptor molecule binds to the concave surface of a donor molecule, which represents a putative interaction site. Mutations in the PRYSPRY domains of Pyrin, which are responsible for familial Mediterranean fever, map on the putative PRYSPRY interaction site.