Studies on the amphid specific glycoprotein gp32 in different life-cycle stages of Meloidogyne species

Abstract

SUMMARY: Indirect immunofluorescent studies have been used to localize a glycoprotein (gp32) in the amphids of 2nd-stage juveniles (J2) of Meloidogyne incognita parasitic in the roots of tomato plants, male Meloidogyne javanica and some young adult female M. incognita. Immuno-electron microscopic studies showed an apparent absence of gp32 in the amphids of mature adult female M. incognita. Chemoattraction experiments demonstrated that the binding of gp32 antibodies to the amphidial secretions of infective J2 M. incognita partly impaired the ability of the nematodes to locate tomato roots.