IL-6 trans-signalling contributes to aldosterone-induced cardiac fibrosis
Open Access
- 19 January 2018
- journal article
- research article
- Published by Oxford University Press (OUP) in Cardiovascular Research
- Vol. 114 (5), 690-702
- https://doi.org/10.1093/cvr/cvy013
Abstract
An excess of aldosterone results in cardiac remodelling and fibrosis. Interleukin-6 (IL-6) is a key mediator in the fibrotic process; however, the effect of aldosterone on the expression of IL-6 remains unclear. We investigated whether aldosterone induces the expression of IL-6 and thereby contributes to the fibrotic process. In this clinical study, we prospectively enrolled 25 patients with primary aldosteronism (PA) and 26 patients with essential hypertension (EH). The PA patients had higher plasma IL-6 levels, left ventricular mass index, degree of myocardial fibrosis, and more impaired diastolic function than the EH patients. In addition, plasma IL-6 levels were positively correlated with 24-h urinary aldosterone and echocardiographic parameters. In cell studies, we investigated the possible molecular mechanism how aldosterone-induced IL-6 secretion and the further effects of collagen production. Aldosterone significantly induced IL-6 protein and mRNA production in human umbilical vein endothelial cells. Intracellular signalling occurred through the mineralocorticoid receptor/PI3K/Akt/NF-kB pathway. In cardiac fibroblasts, IL-6 trans-signalling played a critical role in aldosterone-induced IL-6-enhanced fibrosis-related factor expression. To further investigate the role of IL-6 trans-signalling in aldosterone-induced cardiac fibrosis, we measured the severity of myocardial fibrosis in aldosterone infusion mice models including an IL-6 chemical inhibitor and Sgp130 Knockin Transgenic Mice. Mice receiving recombinant soluble gp130 and Sgp130 Knockin Transgenic Mice prevented myocardial fibrosis and cardiac hypertrophy by aldosterone infusion. IL-6 trans-signalling contributes to aldosterone-induced cardiac fibrosis.Funding Information
- National Taiwan University Hospital (NTUH 105-S3044, and UN105-060)
- Ministry of Science and Technology (MOST 105-2314-B-002 -122 -MY3, MOST 105-2314-B-002 -123, and MOST 106-2314-B-002 -048 -MY3)
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