Structure of a Copper Pump Suggests a Regulatory Role for Its Metal-Binding Domain
Open Access
- 1 June 2008
- journal article
- research article
- Published by Elsevier BV in Structure
- Vol. 16 (6), 976-985
- https://doi.org/10.1016/j.str.2008.02.025
Abstract
P-type ATPases play an important role in Cu homeostasis, which provides sufficient Cu for metalloenzyme biosynthesis but prevents oxidative damage of free Cu to the cell. The PIB group of P-type ATPases includes ATP-dependent pumps of Cu and other transition metal ions, and it is distinguished from other family members by the presence of N-terminal metal-binding domains (MBD). We have determined structures of two constructs of a Cu pump from Archaeoglobus fulgidus (CopA) by cryoelectron microscopy of tubular crystals, which reveal the overall architecture and domain organization of the molecule. By comparing these structures, we localized its N-terminal MBD within the cytoplasmic domains that use ATP hydrolysis to drive the transport cycle. We have built a pseudoatomic model by fitting existing crystallographic structures into the cryoelectron microscopy maps for CopA, which suggest a Cu-dependent regulatory role for the MBD.Keywords
This publication has 67 references indexed in Scilit:
- Rice P1B-Type Heavy-Metal ATPase, OsHMA9, Is a Metal Efflux ProteinPlant Physiology, 2007
- Interdomain communication in calcium pump as revealed in the crystal structures with transmembrane inhibitorsProceedings of the National Academy of Sciences of the United States of America, 2007
- The structure and function of heavy metal transport P1B-ATPasesBioMetals, 2007
- Role of metal-binding domains of the copper pump from Archaeoglobus fulgidusBiochemical and Biophysical Research Communications, 2006
- Structure of human Wilson protein domains 5 and 6 and their interplay with domain 4 and the copper chaperone HAH1 in copper uptakeProceedings of the National Academy of Sciences of the United States of America, 2006
- UCSF Chimera?A visualization system for exploratory research and analysisJournal of Computational Chemistry, 2004
- A structural model for the catalytic cycle of Ca2+-ATPaseJournal of Molecular Biology, 2002
- Structural organization, ion transport, and energy transduction of P-type ATPasesBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1996
- SPIDER and WEB: Processing and Visualization of Images in 3D Electron Microscopy and Related FieldsJournal of Structural Biology, 1996
- Structural basis for E1–E2 conformational transitions in Na, K-pump and Ca-pump proteinsThe Journal of Membrane Biology, 1988