Characterization of Class I Newcastle Disease Virus Isolates from Hong Kong Live Bird Markets and Detection Using Real-Time Reverse Transcription-PCR
- 1 April 2007
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 45 (4), 1310-1314
- https://doi.org/10.1128/jcm.02594-06
Abstract
Newcastle disease viruses isolated from Hong Kong live bird markets (LBMs) were not detected by a USDA-validated matrix gene real-time reverse transcription-PCR (RT-PCR) assay. Based upon phylogenetic analysis of the fusion gene, these viruses were related to lentogenic class I viruses found in U.S. LBMs and wild waterfowl. An alternative real-time RT-PCR assay which complements the matrix gene assay was developed to efficiently detect class I viruses.Keywords
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