Circulating microRNAs as potential markers of human drug-induced liver injury
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Open Access
- 11 July 2011
- journal article
- research article
- Published by Ovid Technologies (Wolters Kluwer Health) in Hepatology
- Vol. 54 (5), 1767-1776
- https://doi.org/10.1002/hep.24538
Abstract
New biomarkers of liver injury are required in the clinic and in preclinical pharmaceutical evaluation. Previous studies demonstrate that two liver‐enriched microRNAs (miR‐122 and miR‐192) are promising biomarkers of acetaminophen‐induced acute liver injury (APAP‐ALI) in mice. We have examined these molecules, for the first time, in humans with APAP poisoning. Serum miR‐122 and miR‐192 were substantially higher in APAP‐ALI patients, compared to healthy controls (median ΔΔCt [25th, 75th percentile]) (miR‐122: 1,265 [491, 4,270] versus 12.1 [7.0, 26.9], P < 0.0001; miR‐192: 6.9 [2.0, 29.2] versus 0.44 [0.30, 0.69], P < 0.0001). A heart‐enriched miR‐1 showed no difference between APAP‐ALI patients and controls, whereas miR‐218 (brain‐enriched) was slightly higher in the APAP‐ALI cohort (0.17 [0.07, 0.50] versus 0.07 [0.04, 0.12]; P = 0.01). In chronic kidney disease (CKD) patients, miR‐122 and ‐192 were modestly higher, compared to controls (miR‐122: 32.0 [21.1, 40.9] versus 12.1 [7.0, 26.9], P = 0.006; miR‐192: 1.2 [0.74, 1.9] versus 0.44 [0.30, 0.69], P = 0.005), but miR‐122 and ‐192 were substantially higher in APAP‐ALI patients than CKD patients (miR‐122: P < 0.0001; miR‐192: P < 0.0004). miR‐122 correlated with peak ALT levels in the APAP‐ALI cohort (Pearson R = 0.46, P = 0.0005), but not with prothrombin time. miR‐122 was also raised alongside peak ALT levels in a group of patients with non‐APAP ALI. Day 1 serum miR‐122 levels were almost 2‐fold higher in APAP‐ALI patients who satisfied King's College Criteria (KCC), compared to those who did not satisfy KCC, although this did not reach statistical significance (P = 0.15). Conclusion: This work provides the first evidence for the potential use of miRNAs as biomarkers of human drug‐induced liver injury. (HEPATOLOGY 2011;)This publication has 27 references indexed in Scilit:
- Assessment of candidate biomarkers of drug-induced hepatobiliary injury in preclinical toxicity studiesToxicology Letters, 2010
- The discovery and development of proteomic safety biomarkers for the detection of drug-induced liver toxicityToxicology and Applied Pharmacology, 2010
- High-Mobility Group Box-1 Protein and Keratin-18, Circulating Serum Proteins Informative of Acetaminophen-Induced Necrosis and Apoptosis In VivoToxicological Sciences, 2009
- Loss of miR-122 expression in liver cancer correlates with suppression of the hepatic phenotype and gain of metastatic propertiesOncogene, 2009
- Circulating microRNAs, potential biomarkers for drug-induced liver injuryProceedings of the National Academy of Sciences of the United States of America, 2009
- miR-122, a paradigm for the role of microRNAs in the liverJournal of Hepatology, 2008
- Characterization of microRNA expression profiles in normal human tissuesBMC Genomics, 2007
- Acetaminophen-induced acute liver failure: Results of a United States multicenter, prospective studyHepatology, 2005
- Acetaminophen and the U.S. acute liver failure study group: Lowering the risks of hepatic failureHepatology, 2004
- MicroRNAs: Genomics, Biogenesis, Mechanism, and FunctionCell, 2004