Do the higher oxidation states of the photosynthetic O2‐evolving system contain bound H2O?
- 20 January 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 195 (1-2), 285-289
- https://doi.org/10.1016/0014-5793(86)80178-8
Abstract
A modified mass spectrometer was used to determine whether the higher oxidation states of the photosynthetic O2-evolving system contain substrate water that is not freely exchangeable with the external medium. Our data indicated that the higher oxidation states contain no appreciable bound, non-exchangeable H2O. This suggests that H2O oxidation takes place via a rapid, concerted, all-or-none mechanism rather than by a mechanism involving stable, partially oxidized, H2O-derived intermediates. These findings set definite constraints on possible mechanisms of O2 evolutionKeywords
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