The production of myco-diesel hydrocarbons and their derivatives by the endophytic fungus Gliocladium roseum (NRRL 50072)
- 1 November 2008
- journal article
- Published by Microbiology Society in Microbiology
- Vol. 154 (11), 3319-3328
- https://doi.org/10.1099/mic.0.2008/022186-0
Abstract
An endophytic fungus, Gliocladium roseum (NRRL 50072), produced a series of volatile hydrocarbons and hydrocarbon derivatives on an oatmeal-based agar under microaerophilic conditions as analysed by solid-phase micro-extraction (SPME)-GC/MS. As an example, this organism produced an extensive series of the acetic acid esters of straight-chained alkanes including those of pentyl, hexyl, heptyl, octyl, sec-octyl and decyl alcohols. Other hydrocarbons were also produced by this organism, including undecane, 2,6-dimethyl; decane, 3,3,5-trimethyl; cyclohexene, 4-methyl; decane, 3,3,6-trimethyl; and undecane, 4,4-dimethyl. Volatile hydrocarbons were also produced on a cellulose-based medium, including heptane, octane, benzene, and some branched hydrocarbons. An extract of the host plant, Eucryphia cordifolia (ulmo), supported the growth and hydrocarbon production of this fungus. Quantification of volatile organic compounds, as measured by proton transfer mass spectrometry (PTR-MS), indicated a level of organic substances in the order of 80 p.p.m.v. (parts per million by volume) in the air space above the oatmeal agar medium in an 18 day old culture. Scaling the PTR-MS profile the acetic acid heptyl ester was quantified (at 500 p.p.b.v.) and subsequently the amount of each compound in the GC/MS profile could be estimated; all yielded a total value of about 4.0 p.p.m.v. The hydrocarbon profile of G. roseum contains a number of compounds normally associated with diesel fuel and so the volatiles of this fungus have been dubbed ‘myco-diesel’. Extraction of liquid cultures of the fungus revealed the presence of numerous fatty acids and other lipids. All of these findings have implications in energy production and utilization.Keywords
This publication has 18 references indexed in Scilit:
- On-Line Monitoring of Microbial Volatile Metabolites by Proton Transfer Reaction-Mass SpectrometryApplied and Environmental Microbiology, 2008
- Potential of the volatile-producing fungus Muscodor albus for control of building moldsCanadian Journal of Microbiology, 2007
- A review on microbial synthesis of hydrocarbonsProcess Biochemistry, 2006
- The α-effect in iminium ion catalysisTetrahedron, 2005
- Asymmetric transesterification of secondary alcohols catalyzed by feruloyl esterase from Humicola insolensBioorganic Chemistry, 2005
- New endophytic isolates of Muscodor albus, a volatile-antibiotic-producing fungusMicrobiology, 2004
- Proton transfer reaction-mass spectrometry as a technique to measure volatile emissions of Muscodor albusPlant Science, 2004
- Biotechnology for the production of commodity chemicals from biomassChemical Society Reviews, 1999
- On-line monitoring of volatile organic compounds at pptv levels by means of proton-transfer-reaction mass spectrometry (PTR-MS) medical applications, food control and environmental researchInternational Journal of Mass Spectrometry and Ion Processes, 1998
- HPLC Separation of Taxol and CephalomannineJournal of Liquid Chromatography, 1991