Purification of ADAM 10 from bovine spleen as a TNFα convertase
Open Access
- 6 January 1997
- journal article
- Published by Wiley in FEBS Letters
- Vol. 400 (3), 333-335
- https://doi.org/10.1016/s0014-5793(96)01410-x
Abstract
We have purified a protease with characteristics of TNFα convertase from bovine spleen membranes. Peptide sequencing of the purified protein identified it as ADAM 10 (Genbank accession no. Z21961). This metalloprotease cleaves a recombinant proTNFα substrate to mature TNFα, and can cleave a synthetic peptide substrate to yield the mature TNFα amino terminus in vitro. The enzyme is sensitive to a hydroxamate inhibitor of MMPs, but insensitive to phosphoramidon. In addition, cloned ADAM 10 mediates proTNFα processing in a processing‐incompetent cell line.Keywords
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