Hydroxyl radical generation in skeletal muscle atrophied by immobilization
- 1 August 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 349 (2), 169-172
- https://doi.org/10.1016/0014-5793(94)00641-5
Abstract
Male Wistar rats (15 weeks old), the ankle joints of one hindlimb of which were immobilized in the extended position for 12 days, were injected with salicylate as a trapping agent for hydroxyl radicals before sacrifice. Atrophied and contralateral soleus, typical slow red muscles were collected and their levels of 2,3-dihydroxybenzoic acid (2,3-DHB), one of the main reaction products formed by the attack of hydroxyl radical on salicylate, were determined using high-performance liquid chromatography with an electrochemical detector. There was a significant increase in 2,3-DHB in the atrophied muscle. This result strongly suggests the enhanced generation in vivo of hydroxyl radicals in atrophied muscle.Keywords
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