Functional analysis of a carboxylesterase gene involved in beta‐cypermethrin and phoxim resistance in Plutella xylostella (L.)
- 20 December 2020
- journal article
- research article
- Published by Wiley in Pest Management Science
- Vol. 77 (4), 2097-2105
- https://doi.org/10.1002/ps.6238
Abstract
BACKGROUND Carboxylesterases (CarEs) are associated with detoxification of xenobiotics, including insecticides, in organism bodies. Overexpression of CarE genes is considered to have an important role in insecticide resistance in insects, however its involvement in multi‐insecticide resistance has rarely been reported. This study aimed to assess the function of a CarE gene (PxαE8) in resistance to five insecticides in Plutella xylostella. RESULTS Relative expression of PxαE8 in three multi‐insecticide‐resistant Plutella xylostella populations, GD‐2017, GD‐2019 and HN, was14.8‐, 19.5‐ and 28.0‐fold higher than that in the susceptible population. Exposure to lethal concentrations associated with 25% mortality (LC25) of beta‐cypermethrin, chlorantraniliprole, metaflumizone, phoxim and tebufenozide could induce the specific activity of CarEs and increase the relative expression of PxαE8. By contrast, knockdown of PxαE8 expression dramatically reduced the activity of CarEs and increased the resistance of P. xylostella (GD‐2019) larvae to beta‐cypermethrin and phoxim by 47.4% and 45.5%, respectively. Further, a transgenic line of Drosophila melanogaster overexpressing PxαE8 was constructed and the bioassay results showed that the tolerance of transgenic Drosophila to beta‐cypermethrin and phoxim was 3.93‐ and 3.98‐fold higher than that of the untransgenic line. CONCLUSION These results provide evidence that overexpression of PxαE8 is involved in resistance, at least to beta‐cypermethrin and phoxim, in multi‐insecticide‐resistant P. xylostella populations, which could help in further understanding the molecular mechanisms of multi‐insecticide resistance in this pest.Keywords
Funding Information
- National Natural Science Foundation of China (31772186, 31572023, 31371956)
This publication has 50 references indexed in Scilit:
- Structure and function of an insect alpha-carboxylesterase (alpha Esterase7) associated with insecticide resistanceProceedings of the National Academy of Sciences of the United States of America, 2013
- Diamondback Moth Ecology and Management: Problems, Progress, and ProspectsAnnual Review of Entomology, 2013
- Estimating the Economic Cost of One of the World's Major Insect Pests, Plutella xylostella (Lepidoptera: Plutellidae): Just How Long Is a Piece of String?Journal of Economic Entomology, 2012
- Two single mutations commonly cause qualitative change of nonspecific carboxylesterases in insectsInsect Biochemistry and Molecular Biology, 2011
- Overexpressed esterases in a fenvalerate resistant strain of the cotton bollworm, Helicoverpa armigeraInsect Biochemistry and Molecular Biology, 2011
- Genomics‐based approaches to screening carboxylesterase‐like genes potentially involved in malathion resistance in oriental migratory locust (Locusta migratoria manilensis)Pest Management Science, 2010
- Characterisation of abamectin resistance in a field‐evolved multiresistant population of Plutella xylostellaPest Management Science, 2009
- Molecular Mechanisms of Metabolic Resistance to Synthetic and Natural XenobioticsAnnual Review of Entomology, 2007
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976