Proteome analysis of glandular parotid and submandibular‐sublingual saliva in comparison to whole human saliva by two‐dimensional gel electrophoresis
- 2 March 2006
- journal article
- research article
- Published by Wiley in Proteomics
- Vol. 6 (5), 1631-1639
- https://doi.org/10.1002/pmic.200500125
Abstract
The secretions of the salivary parotid and submandibular‐sublingual (SMSL) glands constitute the main part of whole human saliva (WS) in which proline‐rich proteins (PRPs) and mucins represent dominant groups. Although proteome analysis had been performed on WS, no identification of PRPs or mucins by 2‐DE and MS was achieved in WS and no comprehensive analysis of both glandular secretions is available so far. The aim of this study was to compare the protein map of WS to parotid and SMSL secretions for the display of PRPs and mucins. WS and glandular secretions were subjected to 2‐DE and spots were analyzed by MALDI‐MS. New components identified in WS were cyclophilin‐B and prolyl‐4‐hydroxylase. Also acidic and basic PRPs as well as the proline‐rich glycoprotein (PRG) could now be mapped in WS. Acidic PRPs were found equally in parotid and SMSL secretions, whereas basic PRPs and PRG were found primarily in parotid secretion. Salivary mucin MUC7 was identified in SMSL secretion. Thus, the more abundant proteins of WS can be explained mainly by mixed contributions of parotid and SMSL secretions with only few components remaining that may be derived from local sources in the oral cavity.Keywords
This publication has 43 references indexed in Scilit:
- Application of fluorescence difference gel electrophoresis saturation labelling for the analysis of microdissected precursor lesions of pancreatic ductal adenocarcinomaProteomics, 2005
- Two-Dimensional Liquid Chromatography Study of the Human Whole Saliva ProteomeJournal of Proteome Research, 2004
- Salivary Proteins: Protective and Diagnostic Value in Cariology?Caries Research, 2004
- Identification of human whole saliva protein components using proteomicsProteomics, 2004
- Two‐dimensional electrophoresis replica blotting: A valuable technique for the immunological and biochemical characterization of single components of complex extractsProteomics, 2003
- Mapping of proteins in human saliva using two‐dimensional gel electrophoresis and peptide mass fingerprintingProteomics, 2003
- In Defense of the Oral Cavity: Structure, Biosynthesis, and Function of Salivary MucinsAnnual Review of Physiology, 1995
- The Role of Salivary Measurements in the Diagnosis of Salivary Autoimmune DiseasesAnnals of the New York Academy of Sciences, 1993
- Two‐dimensional electrophoresis of human parotid salivary proteins from normal and connective tissue disorder subjects using immobilised pH gradientsElectrophoresis, 1991
- Structural Aspects of Salivary GlycoproteinsJournal of Dental Research, 1987