Molecular architecture of the multisubunit homotypic fusion and vacuole protein sorting (HOPS) tethering complex
- 25 January 2012
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 109 (6), 1991-1996
- https://doi.org/10.1073/pnas.1117797109
Abstract
Membrane fusion within the eukaryotic endomembrane system depends on the initial recognition of Rab GTPase on transport vesicles by multisubunit tethering complexes and subsequent coupling to SNARE-mediated fusion. The conserved vacuolar/lysosomal homotypic fusion and vacuole protein sorting (HOPS) tethering complex combines both activities. Here we present the overall structure of the fusion-active HOPS complex. Our data reveal a flexible approximate to 30-nm elongated seahorse-like structure, which can adopt contracted and elongated shapes. Surprisingly, both ends of the HOPS complex contain a Rab-binding subunit: Vps41 and Vps39. The large head contains in addition to Vps41 the SNARE-interacting Vps33, whereas Vps39 is found in the bulky tip of its tail. Vps11 and Vps18 connect head and tail. Our data suggest that HOPS bridges Ypt7-positive membranes and chaperones SNAREs at fusion sites.This publication has 44 references indexed in Scilit:
- HOPS drives vacuole fusion by binding the vacuolar SNARE complex and the Vam7 PX domain via two distinct sitesMolecular Biology of the Cell, 2011
- Tethering Factors as Organizers of Intracellular Vesicular TrafficAnnual Review of Cell and Developmental Biology, 2010
- Defined Subunit Arrangement and Rab Interactions Are Required for Functionality of the HOPS Tethering ComplexTraffic, 2010
- HOPS Initiates Vacuole Docking by Tethering Membranes before trans-SNARE Complex AssemblyMolecular Biology of the Cell, 2010
- A Structure-Based Mechanism for Vesicle Capture by the Multisubunit Tethering Complex Dsl1Cell, 2009
- The Major Role of the Rab Ypt7p in Vacuole Fusion Is Supporting HOPS Membrane AssociationOnline Journal of Public Health Informatics, 2009
- Structural Evidence for Common Ancestry of the Nuclear Pore Complex and Vesicle CoatsScience, 2008
- Reconstituted membrane fusion requires regulatory lipids, SNAREs and synergistic SNARE chaperonesThe EMBO Journal, 2008
- The CORVET Tethering Complex Interacts with the Yeast Rab5 Homolog Vps21 and Is Involved in Endo-Lysosomal BiogenesisDevelopmental Cell, 2007
- Negative staining and image classification — powerful tools in modern electron microscopyBiological Procedures Online, 2004