Visualization and analysis of gene expression in tissue sections by spatial transcriptomics
Top Cited Papers
- 1 July 2016
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 353 (6294), 78-82
- https://doi.org/10.1126/science.aaf2403
Abstract
Analysis of the pattern of proteins or messengerRNAs (mRNAs) in histological tissue sections is a cornerstone in biomedical research and diagnostics. This typically involves the visualization of a few proteins or expressed genes at a time. We have devised a strategy, which we call “spatial transcriptomics,” that allows visualization and quantitative analysis of the transcriptome with spatial resolution in individual tissue sections. By positioning histological sections on arrayed reverse transcription primers with unique positional barcodes, we demonstrate high-quality RNA-sequencing data with maintained two-dimensional positional information from the mouse brain and human breast cancer. Spatial transcriptomics provides quantitative gene expression data and visualization of the distribution of mRNAs within tissue sections and enables novel types of bioinformatics analyses, valuable in research and diagnostics.Funding Information
- Knut och Alice Wallenberg Foundation
- Swedish Foundation for Strategic Research
- Swedish Cancer Society
- Karolinska Institute
- Tobias Stiftelsen
- Torsten Söderbergs Stiftelse
- Ragnar Söderbergs Stiftelse
- StratRegen
- Åke Wiberg Foundation
- Jeansson Foundations
- Swedish Research Council
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