A new principle for the detection of specific IgM antibodies applied in an ELISA for hepatitis a
- 1 January 1979
- journal article
- research article
- Published by Wiley in Journal of Medical Virology
- Vol. 4 (1), 25-32
- https://doi.org/10.1002/jmv.1890040104
Abstract
A new test principle for the detection of specific IgM‐class antibodies was developed and applied in an Enzyme‐Linked Immuno Sorbent Assay (ELISA) for the detection of hepatitis A IgM antibodies. A solid phase coated with anti IgM was incubated successively with serum sample, specific antigen, and enzymelabeled F (ab')2 fragments from IgG antibodies against the antigen and enzyme substrate. F(ab')2 fragments were used to avoid interference with rheumatoid factor. Specificity and sensitivity are very high. This test principle appears generally applicable in the diagnosis of infectious and parasitic diseases by testing only one serum sample.Keywords
This publication has 9 references indexed in Scilit:
- SPECIFIC DETECTION OF IgM-ANTIBODIES BY ELISA, APPLIED IN HEPATITIS-AThe Lancet, 1978
- ELISA IN HEPATITIS AThe Lancet, 1978
- Elimination of false-positive cytomegalovirus immunoglobulin M-fluorescent-antibody reactions with immunoglobulin M serum fractionsJournal of Clinical Microbiology, 1977
- Modified Staphylococcal Absorption Method Used for Detecting Rubella-Specific Immunoglobulin M Antibodies During a Rubella EpidemicJournal of Clinical Microbiology, 1977
- Solid-phase radioimmunoassay of rubella virus immunoglobulin M antibodies: comparison with sucrose density gradient centrifugation testJournal of Clinical Microbiology, 1977
- Solid-phase enzyme-immunoassay for detection of hepatitis B surface antigen.Journal of Clinical Pathology, 1976
- PEROXIDASE-LABELED ANTIBODY A NEW METHOD OF CONJUGATIONJournal of Histochemistry & Cytochemistry, 1974
- The Igm Antibody Response In Rubella During PregnancyJournal of Medical Microbiology, 1971
- Crystals of Fragment Fab′: Preparation from Pepsin Digests of Human IgG Myeloma ProteinsNature, 1969