Rapid Surface Plasmon Resonance Immunoassay for the Determination of Deoxynivalenol in Wheat, Wheat Products, and Maize-Based Baby Food
- 22 July 2010
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 58 (16), 8936-8941
- https://doi.org/10.1021/jf101517s
Abstract
A rapid screening assay (9 min/sample) has been developed and validated for the detection of deoxynivalenol in durum wheat, wheat products, and maize-based baby foods using an SPR biosensor. Through a single laboratory validation, the limits of detection (LOD) for wheat, wheat-based breakfast cereal, and maize-based baby food were 57, 9, and 6 μg/kg, respectively. Intra-assay and interassay precisions were calculated for each matrix at the maximum and half-maximum European Union regulatory limits and expressed as the coefficient of variation (CV). All CVs fell below 10% with the exception of the between-run CV for breakfast cereal. Recoveries at the concentrations tested ranged from 92 to 115% for all matrices. Action limits of 161, 348, and 1378 μg/kg were calculated for baby food, wheat-based breakfast cereal, and wheat, respectively, and the linear range of the assay was determined as 250−2000 μg/kg.This publication has 25 references indexed in Scilit:
- A rapid optical immunoassay for the screening of T-2 and HT-2 toxin in cereals and maize-based baby foodTalanta, 2010
- Development and Single-Laboratory Validation of a Pseudofunctional Biosensor Immunoassay for the Detection of the Okadaic Acid Group of ToxinsAnalytical Chemistry, 2009
- Rapid acoustic screening of deoxynivalenol (DON) in grainWorld Mycotoxin Journal, 2008
- Lateral-flow colloidal gold-based immunoassay for the rapid detection of deoxynivalenol with two indicator rangesAnalytica Chimica Acta, 2008
- Development and validation of a liquid chromatography/tandem mass spectrometric method for the determination of 39 mycotoxins in wheat and maizeRapid Communications in Mass Spectrometry, 2006
- Biosensors and multiple mycotoxin analysisFood Control, 2003
- Improved Methods for Conjugating Selected Mycotoxins to Carrier Proteins and Dextran for ImmunoassaysJournal of Agricultural and Food Chemistry, 1995
- Production of Monoclonal Antibodies for the Specific Detection of Deoxynivalenol and 15-Acetyldeoxynivalenol by ELISAJournal of Agricultural and Food Chemistry, 1995
- Enzyme-linked immunosorbent assay employing monoclonal antibody specific for deoxynivalenol (vomitoxin) and several analogsJournal of Agricultural and Food Chemistry, 1988