Practical innovations for high-throughput amplicon sequencing
Top Cited Papers
- 1 September 2013
- journal article
- Published by Springer Science and Business Media LLC in Nature Methods
- Vol. 10 (10), 999-1002
- https://doi.org/10.1038/nmeth.2634
Abstract
We describe improvements for sequencing 16S ribosomal RNA (rRNA) amplicons, a cornerstone technique in metagenomics. Through unique tagging of template molecules before PCR, amplicon sequences can be mapped to their original templates to correct amplification bias and sequencing error with software we provide. PCR clamps block amplification of contaminating sequences from a eukaryotic host, thereby substantially enriching microbial sequences without introducing bias.Keywords
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