Evidence of a Monosynaptic Pathway Between Cells of the Ventromedial Medulla and the Motoneuron Pool of the Thoracic Spinal Cord in Rat: Electron Microscopic Analysis of Synaptic Contacts

Abstract

Previous electrophysiological and anatomical data have suggested the existence of a descending pathway from the ventromedial medulla into the thoracic motoneuron pool. However, systematic light and electron microscopic analysis have not yet been done to reveal such a projection. In the present study, the anterograde tracer, Phaseolus vulgaris leucoagglutinin (PHA-L) was injected into several discrete regions of the medioventral medulla and descending PHA-L-labelled axons were investigated in the thoracic ventral horn using both light and electron microscopy. Light microscopic analysis of descending projections from 20 distinct areas of the medioventral medulla showed that neurons that project predominantly to the intermediate and ventral regions of the thoracic spinal grey matter are located caudal to the facial nucleus. Monosynaptic contacts were found between axons originating from five distinct regions of the medioventral medulla (containing raphé and/or gigantocellular reticular neurons) and cells in the thoracic motoneuron pool. PHA-L-labelled boutons formed synaptic contacts with large calibre dendrites and with somata. Seventy-two per cent of the investigated 32 boutons appeared to have symmetrical synaptic membrane specializations. The majority of the boutons contained only small, pleomorphic vesicles. Our findings show the existence of a direct monosynaptic pathway between the neurons of the ventromedial medulla and thoracic motor nuclei, providing anatomical support for previous physiological data.