Pretreatment of indian cane molasses for increased production of citric acid
- 1 September 1984
- journal article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 26 (9), 1114-1121
- https://doi.org/10.1002/bit.260260915
Abstract
Surface culture citric acid fermentation was carried out by Aspergillus niger T55, a strain isolated from its natural source, using cane molasses, either untreated or treated by various methods. Citric acid biosynthesis is seriously impaired by both organic and inorganic inhibitors. A combined treatment of molasses with tricalcium phosphate, hydrochloric acid, and Sephadex fractionation minimizes the level of inorganic and organic inhibitors in molasses and increases the production of citric acid (65% weight yield based on total reducing sugar). The optimum level of individual metal ions for citric acid production depends on the concentration of other metals in the medium.Keywords
This publication has 8 references indexed in Scilit:
- Formation of gluconic acid at low pH-values by free and immobilized Aspergillus niger cells during citric acid fermentationApplied Microbiology and Biotechnology, 1982
- Occurrence of an organic inhibitor in cane molasses affecting citric acid production by Aspergillus nigerEnzyme and Microbial Technology, 1981
- Influence of manganese on enzyme synthesis and citric acid accumulation inAspergillus nigerApplied Microbiology and Biotechnology, 1977
- Effect of manganese and other heavy metals on submerged citric acid fermentation of molassesBiotechnology & Bioengineering, 1966
- The Effect of Lipids on Citric Acid Production by an Aspergillus niger MutantJournal of General Microbiology, 1963
- An improved colorimetric method for determining ferrocyanide ion, and its application to molassesThe Analyst, 1960
- Use of Dinitrosalicylic Acid Reagent for Determination of Reducing SugarAnalytical Chemistry, 1959
- Direct Determination of Citric Acid in Milk with an Improved Pyridine-Acetic Anhydride MethodJournal of Dairy Science, 1958