Resuscitation of Escherichia coli VBNC cells depends on a variety of environmental or chemical stimuli
- 20 April 2011
- journal article
- Published by Oxford University Press (OUP) in Journal of Applied Microbiology
- Vol. 110 (6), 1601-1611
- https://doi.org/10.1111/j.1365-2672.2011.05016.x
Abstract
To analyse viable but nonculturable (VBNC) state induction in Escherichia coli and resuscitation of VBNC suspensions in several conditions. VBNC were induced in four media, two temperatures and six strains, but only cells produced at 4°C were able to resuscitate. Resuscitation of 14 VBNC suspensions obtained in several conditions occurred in the presence of supernatants of growing cells, in minimal medium supplemented with amino acids or after temperature change, depending on strain. A limited time period beyond no more resuscitation that could be observed was also confirmed. The supernatants positive effect is suggested to be because of a nonproteinaceous molecule, and a combination of methionine, glutamine, threonine, serine and asparagine could be used as primary mix for resuscitation experiments. Escherichia coli resuscitation was already attempted in several conditions, but it is the first time that a positive result was observed in minimal medium supplemented with amino acids or after temperature change. The role of amino acids in resuscitation is of special interest since was never reported for any species.Keywords
This publication has 31 references indexed in Scilit:
- Induction of Escherichia coli O157:H7 into the viable but non‐culturable state by chloraminated water and river water, and subsequent resuscitationEnvironmental Microbiology Reports, 2009
- Inability of Escherichia coli to resuscitate from the viable but nonculturable stateFEMS Microbiology Ecology, 2007
- Viable but non-culturableListeria monocytogeneson parsley leaves and absence of recovery to a culturable stateJournal of Applied Microbiology, 2007
- Potential pitfalls in the quantitative molecular detection ofEscherichia coliO157:H7 in environmental matricesCanadian Journal of Microbiology, 2006
- Cooperativity Between Different Nutrient Receptors in Germination of Spores of Bacillus subtilis and Reduction of This Cooperativity by Alterations in the GerB ReceptorJournal of Bacteriology, 2006
- Induction and Resuscitation of Viable but Nonculturable Salmonella enterica Serovar Typhimurium DT104†Applied and Environmental Microbiology, 2003
- Concentrations of Copper Thought To Be Toxic to Escherichia coli Can Induce the Viable but Nonculturable ConditionApplied and Environmental Microbiology, 2001
- A matter of bacterial life and deathEMBO Reports, 2001
- Stimulation of bacterial growth by heat-stable, norepinephrine-induced autoinducersFEMS Microbiology Letters, 1999
- Hypervariable DNA fingerprinting in Escherichia coli: minisatellite probe from bacteriophage M13Journal of Bacteriology, 1989