Photothermic regulation of gene expression triggered by laser-induced carbon nanohorns
- 23 April 2012
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 109 (19), 7523-7528
- https://doi.org/10.1073/pnas.1204391109
Abstract
The development of optical methods to control cellular functions is important for various biological applications. In particular, heat shock promoter-mediated gene expression systems by laser light are attractive targets for controlling cellular functions. However, previous approaches have considerable technical limitations related to their use of UV, short-wavelength visible (vis), and infrared (IR) laser light, which have poor penetration into biological tissue. Biological tissue is relatively transparent to light inside the diagnostic window at wavelengths of 650–1,100 nm. Here we present a unique optical biotechnological method using carbon nanohorn (CNH) that transforms energy from diagnostic window laser light to heat to control the expression of various genes. We report that with this method, laser irradiation within the diagnostic window resulted in effective heat generation and thus caused heat shock promoter-mediated gene expression. This study provides an important step forward in the development of light-manipulated gene expression technologies.Keywords
This publication has 54 references indexed in Scilit:
- Diameter and rigidity of multiwalled carbon nanotubes are critical factors in mesothelial injury and carcinogenesisProceedings of the National Academy of Sciences of the United States of America, 2011
- Length-Dependent Retention of Carbon Nanotubes in the Pleural Space of Mice Initiates Sustained Inflammation and Progressive Fibrosis on the Parietal PleuraThe American Journal of Pathology, 2011
- Measurement of the Thermal Conductivity of Carbon Nanotube–Tissue Phantom Composites with the Hot Wire Probe MethodAnnals of Biomedical Engineering, 2011
- Optical properties of breast tumor phantoms containing carbon nanotubes and nanohornsJournal of Biomedical Optics, 2011
- High performance in vivo near-IR (>1 μm) imaging and photothermal cancer therapy with carbon nanotubesNano Research, 2010
- Long-term survival following a single treatment of kidney tumors with multiwalled carbon nanotubes and near-infrared radiationProceedings of the National Academy of Sciences of the United States of America, 2009
- Fabrication of ZnPc/protein nanohorns for double photodynamic and hyperthermic cancer phototherapyProceedings of the National Academy of Sciences of the United States of America, 2008
- Carbon nanotubes introduced into the abdominal cavity of mice show asbestos-like pathogenicity in a pilot studyNature Nanotechnology, 2008
- A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applicationsNature Biotechnology, 2002
- The CF mouse: an important tool for studying cystic fibrosisExpert Reviews in Molecular Medicine, 2001