Neuroendocrine changes in rat stomach during experimental diabetes mellitus
- 1 May 1992
- journal article
- research article
- Published by Springer Science and Business Media LLC in Digestive Diseases and Sciences
- Vol. 37 (5), 751-756
- https://doi.org/10.1007/bf01296434
Abstract
The effects of 48 days of streptozotocin-induced diabetes mellitus in rats on plasma concentrations of gastrin, somatostatin, pancreatic glucagon, and enteroglucagon have been assessed. In addition, neuroendocrine changes in sections of gastric mucosa were quantified using a computer-assisted morphometric system following immunohistochemical staining with polyclonal antibodies directed against gastrin, PGP 9.5 (a neural protein), and somatostatin. Diabetes resulted in significantly increased fasting plasma concentrations of somatostatin, and entero- and pancreatic glucagon. In contrast, lower plasma gastrin concentrations and decreased antral G-cell density were noted in diabetic rats. Gastric somatostatin and neuronal PGP 9.5 stain densities were unaltered by diabetes. Stomachs of diabetic rats weighed less, but both the jejunum and ileum showed evidence of mucosal hyperplasia. The gastric neuroendocrine atrophy observed in diabetes may be a consequence of elevated plasma somatostatin derived from nongastric sources. The enhanced growth of the intestinal mucosa may be related, directly or indirectly, to raised intraluminal glucose concentration in diabetes.Keywords
This publication has 26 references indexed in Scilit:
- Delayed stomach to caecum transit time in the diabetic rat. Possible role of hyperglucagonaemia.Gut, 1990
- Compliance changes of the gastrointestinal tract in streptozotocin-induced diabetic rats.The Japanese Journal of Physiology, 1989
- Hyperenteroglucagonaemia and small intestinal mucosal growth after colonic perfusion of glucose in rats.Gut, 1985
- Diarrhea in streptozocin-treated rats. Loss of adrenergic regulation of intestinal fluid and electrolyte transport.JCI Insight, 1985
- The effect of altered luminal nutrition on cellular proliferation and plasma concentrations of enteroglucagon and gastrin after small bowel resection in the ratBritish Journal of Surgery, 1982
- Response of plasma and tissue levels of enteroglucagon immunoreactivity to intestinal resection, lactation and hyperphagiaLife Sciences, 1981
- Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures.Journal of Histochemistry & Cytochemistry, 1981
- Diabetes and the gut.Gut, 1978
- Structural and functional studies on the transformation of the intestinal mucosa in rats with experimental diabetesZeitschrift für Die Gesamte Experimentelle Medizin, 1977
- Small intestinal transit in diabetics.BMJ, 1976