Study of Polytopic Membrane Protein Topological Organization as a Function of Membrane Lipid Composition
Open Access
- 25 January 2010
- book chapter
- protocol
- Published by Springer Science and Business Media LLC in Methods in molecular biology (Clifton, N.J.)
- Vol. 619, 79-101
- https://doi.org/10.1007/978-1-60327-412-8_5
Abstract
A protocol is described using lipid mutants and thiol-specific chemical reagents to study lipid-dependent and host-specific membrane protein topogenesis by the substituted-cysteine accessibility method as applied to transmembrane domains (SCAM™). SCAM™ is adapted to follow changes in membrane protein topology as a function of changes in membrane lipid composition. The strategy described can be adapted to any membrane system.This publication has 34 references indexed in Scilit:
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