IRE1 encodes a putative protein kinase containing a membrane‐spanning domain and is required for inositol phototrophy in Saccharomyces cerevisiae
- 1 June 1992
- journal article
- Published by Wiley in Molecular Microbiology
- Vol. 6 (11), 1441-1446
- https://doi.org/10.1111/j.1365-2958.1992.tb00864.x
Abstract
A novel gene, IRE1, of Saccharomyces cerevisiae was cloned through genetic complementation of a myo- inositol auxotrophic mutant. The predicted amino acid sequence indicated that IRE1 encodes a protein of 126983 Da with two highly hydrophobic regions, probably a signal sequence and a membrane-spanning region. The carboxy-terminal region of IRE1 showed close sequence similarity to the catalytic domains of protein kinases. Disruption of the IRE1 locus caused myo-inositol auxotrophy. The IRE1 product is very likely a protein kinase required for myo-inositol synthesis.Keywords
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